A new matrix protein family related to the nacreous layer formation of Pinctada fucata
A new matrix protein family related to the nacreous layer formation of Pinctada fucata
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DOI：
10.1016/s0014-5793(99)01387-3
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发表时间：
1999-11-26
期刊：
影响因子：
3.5
通讯作者：
Akera, S
中科院分区：
文献类型：
--
作者：
Samata, T;Hayashi, N;Akera, S
我们分离了一个新的基质蛋白家族（N16），该家族特异于日本合浦珠母贝（Pinctada fucata）的珍珠层，并克隆和鉴定了编码该组分的cDNA。推导的氨基酸序列分析表明，N16与其他蛋白没有明确的同源性。结晶的体外研究表明，N16诱导文石晶体固定在基板上时，但抑制晶体的形成没有它，文石晶体表现出不同于无机形成的片状形态，长时间的孵育间隔导致结晶层高度相似的钠长石层。(C)1999年欧洲生物化学学会联合会。
We have isolated a new matrix protein family (N16) which is specific to the nacreous layer of the Japanese pearl oyster, Pinctada fucata, and have cloned and characterized the cDNAs coding for the components. Analysis of the deduced amino acid sequence revealed that N16 showed no definitive homology,vith other proteins. The in vitro studies of the crystallization clarified that N16 induced aragonite crystals when fixed on the substrate but inhibited crystal formation without it, The aragonite crystals showed platy morphology different from those formed inorganically, and long intervals of incubation resulted in crystalline layers highly similar to the nacreous layer. (C) 1999 Federation of European Biochemical Societies.