Morphological and molecular identification of Eimeria tetartooimia oocysts from a Japanese green pheasant (Galliformes; Phasianidae; Phasianus versicolor) at a zoo in Japan.
Morphological and molecular identification of Eimeria tetartooimia oocysts from a Japanese green pheasant (Galliformes; Phasianidae; Phasianus versicolor) at a zoo in Japan.
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日本一家动物园的日本绿雉（鸡形目；雉科；雉鸡）的艾美耳球虫卵囊的形态学和分子鉴定。
DOI：
10.1007/s00436-021-07197-6
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Sasai K.
中科院分区：
文献类型：
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作者：
Matsubayashi M;Takami K;Kinoshita M;Tsuchida S;Ushida K;Shibahara T;Sasai K.
我们在日本大坂的一个动物园从日本绿色雉鸡（Phasianus versicolor）中检测到了个艾美耳球虫囊。卵囊呈近球形或卵圆形，长17.2（14.7-20.0）μm，宽14.8（13.3-16.7）μm，长/宽（L/W）为1.2（1.0-1.4），每个卵囊有一个极粒。卵囊无残留物和珠孔。孢子囊长9.8（6.7-13.3）μm，宽5.9（4.7-7.3）μ m，长/宽比1.2（1.1-1.4）。与先前发表的值相比，该菌株显示出与分离株ofE的形态相似性。来自其他国家的环颈雉的Teetartooimia。18 S rRNA和线粒体细胞色素c氧化酶亚基I基因的系统发育分析将该分离物置于与鸡艾美耳球虫相关的进化枝中，如E。堆生菌素E布鲁内蒂。虽然还需要进一步的分析，这些信息可以帮助诊断和确定Eimeriaspp的毒力。在野鸡。
We detectedEimeriaoocysts from Japanese green pheasants (Phasianus versicolor) at a zoo in Osaka, Japan. The oocyst isolates were subspherical or ovoidal shaped and measured 17.2 (range 14.7–20.0) μm in length and 14.8 (13.3–16.7) μm in width with a length/width (L/W) ratio of 1.2 (1.0–1.4) and each had one polar granule. The oocysts lacked a residuum and micropyle. Sporocysts measured 9.8 (6.7–13.3) μm in length and 5.9 (4.7–7.3) μm in width, with a L/W ratio of 1.2 (1.1–1.4). Compared to previously published values, this strain shows morphological similarities with an isolate ofE. teetartooimiafrom ring-necked pheasants from other countries. Phylogenetic analysis of the 18S rRNA and mitochondrial cytochrome c oxidase subunit I genes places the isolate in a clade related to chickenEimeriaspp., such asE. acervulinaorE. brunetti. Although further analysis is needed, this information can be helpful for the diagnosis and determination of virulence ofEimeriaspp. in pheasants.