DIRECT MEASUREMENT OF INTERSTITIAL CONVECTION AND DIFFUSION OF ALBUMIN IN NORMAL AND NEOPLASTIC TISSUES BY FLUORESCENCE PHOTOBLEACHING
DIRECT MEASUREMENT OF INTERSTITIAL CONVECTION AND DIFFUSION OF ALBUMIN IN NORMAL AND NEOPLASTIC TISSUES BY FLUORESCENCE PHOTOBLEACHING
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DOI：
10.1073/pnas.86.14.5385
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发表时间：
1989-07-01
影响因子：
11.1
通讯作者：
JAIN, RK
中科院分区：
文献类型：
--
作者：
CHARY, SR;JAIN, RK
通过对流和扩散发生通过组织的间隙空间的大分子运输。运输的对流成分是由间质液流动引起的。迄今为止，还没有直接测量组织中间质液流动的大小或方向。使用光漂白后的荧光恢复，我们已经测量了组织间液的速度和扩散系数的牛血清白蛋白在正常和肿瘤组织生长在一个薄的，透明的窗口，在一只兔子的耳朵。一束清晰的激光束聚焦在荧光浴组织的荧光区内。一个短脉冲的激光照射熄灭了从这个选定区域发出的荧光。监测和分析由于介质内的扩散和对流而导致的荧光的恢复，以得到扩散系数和流体速度的值。平均流体速度约为0.6 μ m/s，白蛋白扩散系数为5.8 ± 1.5。1.3. times. 10-7 cm ~ 2/s和6.3 ±. 1.9. times. 10-7 cm 2/s。间质液流，在一般情况下，直接进入毛细血管后微静脉。在这项研究中获得的结果应该提供动力，进一步调查在正常和病理条件下的各种组织中的扩散和对流。
Macromolecular transport through the interstitial space of a tissue occurs by convection and diffusion. The convective component of transport results from interstitial fluid flow. There have been no direct measurements of the magnitude or direction of interstitial fluid flow in tissues to date. Using fluorescence recovery after photobleaching, we have measured interstitial fluid velocities and the diffusion coefficient of bovine serum albumin in normal and neoplastic tissues grown in a thin, transparent window in the ear of a rabbit. A well-defined laser beam was focused on a region within the interstitium of the fluorescence-bathed tissue. A short pulse of laser irradiation extinguished the fluorescence emanating from this selected region. The recovery of fluorescence due to diffusion and convection within the medium was monitored and analyzed to yield values of the diffusion coefficient and the fluid velocity. The average fluid velocity was about 0.6 .mu.m/s, and albumin diffusion coefficients were 5.8 .+-. 1.3 .times. 10-7 cm2/s and 6.3 .+-. 1.9 .times. 10-7 cm2/s in normal and neoplastic tissues, respectively. The interstitial fluid flow, in general, was directed into postcapillary venules. The results obtained in this study should provide the impetus for further investigation into the diffusion and convection in various tissues under normal and pathological conditions.