Role of nucleoporin induction in releasing an mRNA nuclear export block
Role of nucleoporin induction in releasing an mRNA nuclear export block
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DOI：
10.1126/science.1067861
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发表时间：
2002-02-22
期刊：
影响因子：
56.9
通讯作者：
Fontoura, BMA
中科院分区：
文献类型：
--
作者：
Enninga, J;Levy, DE;Fontoura, BMA
信号介导的核输入和输出通过核孔复合物（NPC）进行。一些NPC成分，如核孔蛋白（Nups） Nup98和Nup96，也与核内部有关。Nup98是水疱性口炎病毒（VSV）基质(M)蛋白介导的信使RNA （mRNA）核输出抑制的靶点。在这里，Nup98和Nup96被干扰素（IFN）上调。当细胞被ifn - γ处理或被编码Nup98和Nup96的互补DNA （cDNA）转染时，M蛋白介导的mRNA核输出抑制被逆转。因此，Nup98和Nup96表达的增加构成了ifn介导的机制，逆转了M蛋白介导的基因表达抑制。
Signal-mediated nuclear import and export proceed through the nuclear pore complex (NPC). Some NPC components, such as the nucleoporins (Nups) Nup98 and Nup96, are also associated with the nuclear interior. Nup98 is a target of the vesicular stomatitis virus (VSV) matrix (M) protein-mediated inhibition of messenger RNA (mRNA) nuclear export. Here, Nup98 and Nup96 were found to be up-regulated by interferon (IFN). M protein-mediated inhibition of mRNA nuclear export was reversed when cells were treated with IFN-gamma or transfected with a complementary DNA (cDNA) encoding Nup98 and Nup96. Thus, increased Nup98 and Nup96 expression constitutes an IFN-mediated mechanism that reverses M protein-mediated inhibition of gene expression.