Copper-containing mesoporous bioactive glass nanoparticles as multifunctional agent for bone regeneration
Copper-containing mesoporous bioactive glass nanoparticles as multifunctional agent for bone regeneration
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DOI：
10.1016/j.actbio.2017.04.012
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发表时间：
2017-06-01
影响因子：
9.7
通讯作者：
Vitale-Brovarone, Chiara
中科院分区：
文献类型：
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作者：
Bari, Alessandra;Bloise, Nora;Vitale-Brovarone, Chiara
介孔生物活性玻璃（MBG）具有抗菌、促进骨生成和血管生成等功能，其应用越来越受到人们的关注，本文旨在赋予纳米MBG更多的生物功能，二元SiO2-CaO介孔玻璃的骨架用不同浓度的铜离子（2和5%摩尔）改性，通过一锅超声辅助溶胶-凝胶过程。含Cu的MBG（2%mol.）结果表明，Cu-MBG纳米粒具有较高的比表面积（550 m2·g-1）、均匀的介孔孔道（2.6 nm）、显著的体外生物活性和Cu ~（2+）的缓释作用。coli、S.金黄色葡萄球菌、表皮葡萄球菌，以及抑制和分散由S.表皮。所得结果表明，所开发的材料将优异的生物活性和抗菌能力结合在单一多功能剂中，为预防传染病和有效治疗骨缺损提供了有希望的机会。重要性声明为了赋予具有优异生物活性的介孔生物活性玻璃额外的生物学功能，采用超声辅助一锅法合成了纳米晶形式的Cu掺杂介孔SiO2-CaO玻璃（Cu-MBG）。使用不同细菌菌株的细菌活力分析，以及表皮葡萄球菌产生的生物膜的形态学观察，揭示了Cu-MBG和相关离子提取物对细菌生长和生物膜形成/分散的抗微生物有效性，为传统抗生素全身治疗提供了真正的替代方案。所提出的多功能剂代表了骨和软组织再生的有前途的和通用的平台。(C)2017 Acta Materialia Inc.由爱思唯尔有限公司出版。这是一篇在CC BY-NC-ND许可证下的开放获取文章（http：//creativecommons.org/licenses/by-nc-nd/4.0/）。
The application of mesoporous bioactive glasses (MBGs) containing controllable amount of different ions, with the aim to impart antibacterial activity, as well as stimulation of osteogenesis and angiogenesis, is attracting an increasing interest.In this contribution, in order to endow nano-sized MBG with additional biological functions, the framework of a binary SiO2-CaO mesoporous glass was modified with different concentrations of copper ions (2 and 5% mol.), through a one-pot ultrasound-assisted sol-gel procedure. The Cu-containing MBG (2% mol.) showed high exposed surface area (550 m(2) g(-1)), uniform mesoporous channels (2.6 nm), remarkable in vitro bioactive behaviour and sustained release of Cu2+ ions.Cu-MBG nanoparticles and their ionic dissolution extracts exhibited antibacterial effect against three different bacteria strains, E. coli, S. aureus, S. epidermidis, and the ability to inhibit and disperse the biofilm produced by S. epidermidis.The obtained results suggest that the developed material, which combines in single multifunctional agent excellent bioactivity and antimicrobial ability, offers promising opportunities for the prevention of infectious diseases and the effective treatment of bone defects.Statement of SignificanceIn order to endow mesoporous bioactive glass, characterized by excellent bioactive properties, with additional biological functions, Cu-doped mesoporous SiO2-CaO glass (Cu-MBG) in the form of nanoparticles was prepared by an ultra-sound assisted one pot synthesis.Statement of Significance: The analysis of the bacterial viability, using different bacterial strains, and the morphological observation of the biofilm produced by the Staphylococcus epidermidis, revealed the antimicrobial effectiveness of the Cu-MBG and the relative ionic extracts against both the bacterial growth and the biofilm formation/dispersion, providing a true alternative to traditional antibiotic systemic therapies.Statement of Significance: The proposed multifunctional agent represents a promising and versatile platform for bone and soft tissues regeneration. (C) 2017 Acta Materialia Inc. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).