In vitro degradation and release profiles for poly-dl-lactide-poly(ethylene glycol) microspheres containing human serum albumin
In vitro degradation and release profiles for poly-dl-lactide-poly(ethylene glycol) microspheres containing human serum albumin
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DOI：
10.1016/s0168-3659(01)00210-3
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发表时间：
2001-04-02
影响因子：
10.8
通讯作者：
Yuan, ML
中科院分区：
文献类型：
--
作者：
Deng, XM;Zhou, SB;Yuan, ML
以五种不同分子量的PEG为原料，通过开环聚合合成了聚乙二醇（PEG）含量（10%）的聚-丙交酯-聚乙二醇（PELA）嵌段共聚物。在形成双w/o/w乳液的基础上，采用溶剂萃取法制备了含有人血清白蛋白（HSA）的PELA微球。这些微球的体外基质降解和蛋白质释放在磷酸盐缓冲盐水 (PBS)（154 mM，pH 7.43）中进行。通过测量微球质量的损失、聚合物特性粘度的降低、降解介质pH值的降低、聚合物数均分子量(M-n)的降低和分子量多分散性(M-w/M-n)的变化来表征降解曲线。通过测量不同时间间隔释放介质中存在的蛋白质来研究释放曲线。结果表明，基质降解和蛋白质释放曲线高度依赖于聚合物。随着PELA共聚物分子量的降低，初始蛋白质释放中突释的程度增加。建议这些基质聚合物可以优化为蛋白质（抗原）递送系统中的载体以用于不同的目的。 (C) 2001 Elsevier Science B.V. 保留所有权利。
Poly-dl-lactide-poly(ethylene glycol) (PELA) block copolymers containing same the content (10%) of polyethylene glycol (PEG) were synthesized with five different molecular weight of PEG by ring-opening polymerization. PELA microspheres containing human serum albumin (HSA) were elaborated by solvent extraction method based on the formation of double w/o/w emulsion. In vitro matrix degradation and protein release of these microspheres were performed in phosphate buffered saline (PBS) (154 mM, pH 7.43). The degradation profiles were characterized by measuring the loss of microspheres mass, the decrease of polymer intrinsic viscosity, the decrease of pH value of degradation medium, the reduction of polymer number-average molecular weight (M-n) and the change of molecular weight polydispersity (M-w/M-n). The release profiles were investigated From the measurement of protein presented in the release medium at various intervals. It showed that the matrix degradation and protein release profiles were highly polymer-dependent. The extent of burst release in the initial protein release increased with the decrease of molecular weight of PELA copolymer. It is suggested that these matrix polymers may be optimized as carriers in protein (antigen) delivery system for different purposes. (C) 2001 Elsevier Science B.V. All rights reserved.