Activation of p38 MAPK and expression of TGF-β1 in rat colon enterocytes after whole body γ-irradiation
Activation of p38 MAPK and expression of TGF-β1 in rat colon enterocytes after whole body γ-irradiation
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DOI：
10.3109/09553002.2012.654044
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发表时间：
2012-04-01
影响因子：
2.6
通讯作者：
Kuca, Kamil
中科院分区：
文献类型：
--
作者：
Pejchal, Jaroslav;Novotny, Jakub;Kuca, Kamil
目的：检测p38丝裂原活化蛋白激酶（p38）磷酸化和转化生长因子β 1（TGF β 1）（TGF-β 1）在照射后大鼠结肠肠上皮细胞中的表达及其对肠放射病病理学的贡献。用0.5、1、2、3、4、5、6、7、8、9和10戈伊（Co-60，1.44戈伊·分（-1））γ射线全身照射雄性Wistar大鼠。照射后4 h和24 h分别取标本，进行免疫组化染色，然后用计算机图像分析技术检测根尖和隐窝肠上皮细胞p38磷酸化和TGF-β 1表达。结果：P38磷酸化积分光密度（IOD）水平增加了2.4倍（p < 0.05），而P38磷酸化积分光密度（IOD）水平增加了2.4倍（p < 0.05）。
Purpose : To examine the p38 mitogen-activated protein kinase (p38) phosphorylation and transforming growth factor beta 1 (TGF-beta 1) expression in rat colon enterocytes after irradiation and their contribution to pathology of intestinal radiation disease.Materials and methods : Male Wistar rats were irradiated with whole body gamma-radiation of 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10 Gy (Co-60, 1.44 Gy.min(-1)). Samples were taken 4 and 24 h after irradiation, immunohistochemically stained, then p38 phosphorylation and TGF-beta 1 expression were measured in apical and cryptal enterocytes using computer image analysis. In selected groups, morphometric parameters, mitosis and apoptosis were evaluated.Results : P38 phosphorylation integrated optical density (IOD)-based levels increased 2.4-fold (p