MODIFICATION OF THE GLYOXALASE SYSTEM IN HUMAN RED BLOOD-CELLS BY GLUCOSE INVITRO
MODIFICATION OF THE GLYOXALASE SYSTEM IN HUMAN RED BLOOD-CELLS BY GLUCOSE INVITRO
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DOI：
10.1042/bj2540751
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发表时间：
1988-09-15
影响因子：
4.1
通讯作者：
THORNALLEY, PJ
中科院分区：
文献类型：
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作者：
THORNALLEY, PJ
用体外高浓度葡萄糖孵育的方法对人红细胞谷胱甘肽酶系统进行了修饰。将红细胞悬液（50%，v/v）与5 mM和25 mM葡萄糖一起孵育，以模拟正常和高血糖葡萄糖代谢。随着葡萄糖浓度的增加，丙酮醛通过糖苷酶途径代谢为D-乳酸的通量增加。在研究的范围内（5-100 mM），增加与初始葡萄糖浓度大致成比例。甘氨酰转移酶I和甘氨酰转移酶II的活性没有显著变化，但甘氨酰转移酶底物甲基乙二醛和S-D-乳酰谷胱甘肽的浓度以及通过甘氨酰转移酶途径代谢的葡糖三糖的百分比显著增加。在周期性高血糖期间，通过谷胱甘肽酶途径代谢的中间产物通量增加可能是与糖尿病相关的慢性临床并发症发展相关的生化因素。
The human red-blood cell glyoxalase system was modified by incubation with high concentrations of glucose in vitro. Red-blood-cell suspensions (50%, v/v) were incubated with 5 mM- and 25 mM-glucose to model normal and hyperglycaemic glucose metabolism. There was an increase in the flux of methylglyoxal metabolized to D-lactic acid via the glyoxalase pathway with high glucose concentration. The increase was approximately proportional to initial glucose concentration over the range studied (5-100 mM). The activities of glyoxalase I and glyoxalase II were not significantly changed, but the concentrations of the glyoxalase substrates, methylglyoxal and S-D-lactoylglutathione, and the percentage of glucotriose metabolized via the glyoxalase pathway, were significantly increased. The increase in the flux of intermediates metabolized via the glyoxalase pathway during periodic hyperglycaemia may be a biochemical factor involved in the development of chronic clinical complications associated with diabetes mellitus.