Gold-Photodeposited Silver Nanowire Endoscopy for Cytosolic and Nuclear pH Sensing
Gold-Photodeposited Silver Nanowire Endoscopy for Cytosolic and Nuclear pH Sensing
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用于细胞质和核 pH 传感的金光沉积银纳米线内窥镜检查
DOI：
10.1021/acsanm.1c02363
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发表时间：
2021
影响因子：
5.9
通讯作者：
Uji-i Hiroshi
中科院分区：
文献类型：
--
作者：
Zhang Qiang;Inose Tomoko;Ricci Monica;Li Jiangtao;Tian Ya;Wen Han;Toyouchi Shuichi;Fron Eduard;Ngoc Dao Anh Thi;Kasai Hitoshi;Rocha Susana;Hirai Kenji;Fortuni Beatrice;Uji-i Hiroshi
细胞内pH变化是生理和病理条件的重要指标。因此，已知癌症与pH失调有直接的相互作用。为了研究细胞中的pH变化，由于其高pH灵敏度，金属纳米颗粒已被广泛用作基于表面增强拉曼散射（Sers）的传感器。然而，这些Sers探针允许仅在细胞的酸性隔室（内溶酶体）处检测pH，其中颗粒在其内吞作用后被截留。因此，用金属纳米颗粒获得的结果是有限的，并且在细胞中检测到的pH值与其生理条件之间的关系仍然不清楚。在此，我们提出了一种替代方法的基础上金沉积银纳米线内窥镜研究细胞质和核的pH值的变化，具有高时空分辨率和灵敏度。传感探针的制备是通过沉积金纳米结构的银纳米线（Au-dep银纳米线），通过可见激光照射和修改的表面与pH值响应的拉曼报告（4-巯基苯甲酸）。通过将探针浸入不同pH值（4.4-9.3）的溶液中，证明了高pH灵敏度。然后将内窥镜探针插入活HeLa细胞的细胞核或细胞溶质中，用于位点特异性pH传感。在分别添加缺氧模拟剂（CoCl 2）和抗癌药物（顺铂）后进行相同的实验。值得注意的是，我们的探针准确地检测到这些处理随时间的特定pH变化。在未处理的细胞中未测量到类似的pH变化。在这项工作中报告的结果清楚地表明，Au-dep AgNW内窥镜是一个有前途的强大的工具，在生物系统中的pH传感应用。
Intracellular pH variations are a crucial indicator of physiological and pathological conditions. As such, cancer is known to have a direct interplay with pH dysregulation. For investigation of the pH alterations in cells, metal nanoparticles have been widely used as surface-enhanced Raman scattering (SERS)-based sensors thanks to their high pH sensitivity. However, these SERS probes allow for detection of the pH exclusively at the acidic compartments of the cells (endolysosomes), where particles are entrapped after their endocytosis. Consequently, the results obtained with metal nanoparticles are limited, and the relationship between the pH values detected in the cells and their physiological conditions remains unclear. Herein, we propose an alternative approach based on gold-deposited silver nanowire endoscopy to study cytosolic and nuclear pH variations with high spatiotemporal resolution and sensitivity. The sensing probe was fabricated by depositing gold nanostructures on silver nanowires (Au-dep AgNWs) via visible-laser-light irradiation and modifying the surface with a pH-responsive Raman reporter (4-mercaptobenzoic acid). The high pH sensitivity was demonstrated by immersing the probe in solutions with different pH values (4.4–9.3). The endoscopic probe was then inserted into either the nucleus or cytosol of a living HeLa cell for site-specific pH sensing. The same experiments were performed after the addition of a hypoxia mimetic agent (CoCl2) and an anticancer drug (cisplatin), individually. Notably, our probe accurately detected specific pH variations upon these treatments over time. Similar pH alterations were not measured in untreated cells. The results reported in this work clearly show that Au-dep AgNW endoscopy is a promising powerful tool for pH-sensing applications in biological systems.