Immobilization of Treponema pallidum in vitro by antibody produced in syphilitic infection.
Immobilization of Treponema pallidum in vitro by antibody produced in syphilitic infection.
复制标题
梅毒感染中产生的抗体在体外固定颗粒。
DOI：
10.1084/jem.89.4.369
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发表时间：
1949-04-01
影响因子：
15.3
通讯作者：
MAYER, M M
中科院分区：
文献类型：
--
作者：
NELSON, R A Jr;MAYER, M M
从兔睾丸梅毒瘤中提取梅毒螺旋体，并将其悬浮在特殊培养基中，使其保持活力和传染性数天。将这种悬浮液与梅毒兔或人血清和豚鼠补体孵育后，密螺旋体变得不活动，失去了感染兔子的能力。研究了影响这种固定的各种因素。在对个体血清的初步调查中，在大多数梅毒动物和人的血清中检测到固定抗体，而在几乎所有的正常血清中检测不到固定抗体。这表明梅毒血清的固定化和再激活活性是由不同的抗体引起的。
Treponema pallida were extracted from rabbit testicular syphilomas and suspended in a special medium in which the organisms remain motile and infectious for several days. On incubation of such suspensions with syphilitic rabbit or human sera and guinea pig complement, the treponemes became non-motile and lost their capacity to infect rabbits. Various factors affecting this immobilization have been investigated. In a preliminary survey of individual sera, immobilizing antibody could be detected in the majority of sera from syphilitic animals and human beings, but was absent in almost all the normal sera examined. It could be demonstrated that the immobilizing and reagin activities of syphilis sera are due to separate antibodies.