Periostin antisense oligonucleotide prevents adhesion formation after surgery in mice
Periostin antisense oligonucleotide prevents adhesion formation after surgery in mice
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Periostin反义寡核苷酸可预防小鼠手术后粘连形成
DOI：
10.1016/j.jphs.2016.10.009
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发表时间：
2017
影响因子：
3.5
通讯作者：
Jin Denan
中科院分区：
文献类型：
--
作者：
Takai Shinji;Yoshino Masafumi;Takao Kazumasa;Yoshikawa Kazunori;Jin Denan
为了研究骨膜蛋白在粘连形成中的作用，在小鼠中评价骨膜蛋白反义寡核苷酸（PAO）对粘连形成的影响。在麻醉下刮除盲肠浆膜，术后测定粘连评分和骨膜蛋白及其相关因子的mRNA水平。术后腹腔注射生理盐水、40 mg/kg负义寡核苷酸（NSO）或40 mg/kg PAO，14 d后评价粘连评分和mRNA水平。术后1天观察到薄膜粘连形成，粘连评分逐渐增加至14天。骨膜蛋白、转化生长因子-β（TGF-β）和I型胶原mRNA水平从3天到14天逐渐升高。术后14天PAO组的粘连评分明显低于生理盐水组和NSO组。与生理盐水或NSO相比，用PAO处理的骨膜蛋白、TGF-β和胶原I的mRNA水平也显著减弱。因此，这些结果表明，骨膜蛋白mRNA水平在磨损的盲肠中增加，并且PAO沿着骨膜蛋白mRNA水平的衰减防止粘连形成。
To study the role of periostin in adhesion formation, the effect of periostin antisense oligonucleotide (PAO) on adhesion formation was evaluated in mice. Under anesthesia, the serous membrane of the cecum was abraded, and the adhesion score and mRNA levels of periostin and its related factors were determined after surgery. Saline, 40 mg/kg of negative sense oligonucleotide (NSO), or 40 mg/kg of PAO were injected into the abdomen after surgery, and the adhesion score and mRNA levels were evaluated 14 days later. Filmy adhesion formation was observed 1 day after surgery, and the adhesion score increased gradually to 14 days. The mRNA levels of periostin, transforming growth factor (TGF)-β, and collagen I increased gradually from 3 days to 14 days. The adhesion score of PAO was significantly lower than of saline or NSO 14 days after surgery. The mRNA levels of periostin, TGF-β, and collagen I were also significantly attenuated by treatment with PAO compared with saline or NSO. Thus, these results demonstrated that the periostin mRNA level increased in the abraded cecum, and PAO prevented adhesion formation along with attenuation of the periostin mRNA level.