HIV phenotype correlates with the relative amounts of lymphocyte function-related molecule 1 (LFA-1) and major histocompatibility complex (MHC) class II in the virion envelope.
HIV phenotype correlates with the relative amounts of lymphocyte function-related molecule 1 (LFA-1) and major histocompatibility complex (MHC) class II in the virion envelope.
复制标题
HIV 表型与病毒颗粒包膜中淋巴细胞功能相关分子 1 (LFA-1) 和主要组织相容性复合体 (MHC) II 类的相对量相关。
DOI：
10.1097/00002030-200007280-00008
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Zolla-Pazner,S
中科院分区：
文献类型：
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作者：
BastianiLallos,L;Cecilia,D;Fenyö,EM;Laal,S;Zolla-Pazner,S
HIV-1的生物学表型与其感染性的各个方面有关，包括合胞体形成和辅助受体的使用。存在于靶细胞和病毒上的粘附分子也已被证明在感染过程中发挥作用。为探讨HIV-1包膜上粘附分子的存在与病毒生物学表型之间的可能关系，对56株已知生物学表型的HIV-1分离株的包膜进行了淋巴细胞功能相关分子1（LFA-1）和主要组织相容性复合物（MHC）的分析。在GHOST细胞或U87中测定每种分离物的辅助受体使用。CD 4感染性测定。结果与使用趋化因子受体CXCR 4的病毒相比，使用趋化因子受体CCR 5的病毒在其包膜中的MHC II类分子水平相对高于使用趋化因子受体CXCR 4的病毒。使用病毒增加了对HIV-1的生物表型有贡献的特性的列表。这可以部分解释使用CXCR 4的病毒如何能够比使用CCR 5的病毒结合并感染更广泛的细胞类型，以及为什么使用CXCR 4的病毒与更具侵略性的疾病过程相关。
ObjectiveThe biological phenotype of HIV-1 has been associated with various aspects of its infectivity, including syncytium formation and coreceptor usage. Adhesion molecules, present on both the target cell and the virus, have also been shown to play a role in the infectious process. A possible correlation between the presence of adhesion molecules in the envelope of HIV-1 with the biological phenotype of the virus is examined.DesignThe envelopes of 56 isolates of HIV-1 of known biological phenotype were analyzed for the presence of lymphocyte function-related molecule 1 (LFA-1) and major histocompatibility complex (MHC) class II molecules.MethodsThe coreceptor usage of each isolate was determined in a GHOST cell or a U87. CD4 infectivity assay. The presence of LFA-1 and MHC class II in each virus envelope was then determined using a virus-binding enzyme-linked immunosorbent assay (ELISA).ResultsViruses using the chemokine receptor CCR5 have relatively higher levels of MHC class II than LFA-1 in their envelopes compared with those using CXCR4.ConclusionsThe finding that there is a differential incorporation of MHC class II and LFA-1 molecules by CXCR4-and CCR5-using viruses augments the list of properties contributing to the biological phenotype of HIV-1. This may explain, in part, how CXCR4-using viruses are able to bind to and infect a broader range of cell types than CCR5-using viruses, and why CXCR4-using viruses are associated with a more aggressive disease course.