[H-3] NISOXETINE BINDING-SITES IN THE CAT BRAIN - AN AUTORADIOGRAPHIC STUDY
[H-3] NISOXETINE BINDING-SITES IN THE CAT BRAIN - AN AUTORADIOGRAPHIC STUDY
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DOI：
10.1016/0306-4522(95)00257-j
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发表时间：
1995-11-01
期刊：
影响因子：
3.3
通讯作者：
BOURAS, C
中科院分区：
文献类型：
--
作者：
CHARNAY, Y;LEGER, L;BOURAS, C
[H-3]nisoxetine，高亲和力的去甲肾上腺素摄取位点的选择性抑制剂的结合，研究了猫脑额叶冷冻切片。蓝斑核及其上行通路、最后区和迷走神经运动背核放射自显影信号密度最高。其他显示高密度信号的区域包括下橄榄核的背侧部、脑桥核、中缝核、丘、下丘的室周和外侧区、视交叉上核、延髓核和嗅球。在海马结构，特别是在分子层的阿蒙的角，内嗅皮层的浅层和在灰盖中观察到一个中等高浓度的结合位点。在新皮层的所有分区中观察到结合位点。最高密度的结合通常在表面层I的外缘检测到。在某些皮质区，特别是在视觉皮层，标记与流行的层状分布在浅层I-III和深层V-VI清楚地观察到。中度至低密度的结合位点被认为是在大多数其他地区的大脑，除了在白色的问题，尾状核和壳核，这是缺乏labeling.Overall这些研究结果表明之间的良好的相关性[H-3]nisoxetine结合位点的分布和去甲肾上腺素能系统。此外，数据表明，在几个领域，高亲和力的去甲肾上腺素再摄取机制可以发挥重要作用，在当地的去甲肾上腺素能系统和其他单胺能系统之间的相互作用。
The binding of [H-3]nisoxetine, a selective inhibitor of the high-affinity noradrenaline uptake sites, was studied on frontal frozen sections of the cat brain. The highest densities in autoradiographic signal were observed in the nucleus locus coeruleus and its ascending pathways, in the area postrema and in the dorsal motor nucleus of the vagus nerve. Other areas displaying high densities of signal include the dorsal part of the inferior olive, the pontine nuclei, the raphe nuclei, the colliculi, the periventricular and lateral areas of the hypothalamus, the suprachiasmatic nucleus, the nucleus accumbens and the olfactory bulb. A moderately high concentration of binding sites was observed in the hippocampal formation, especially in the molecular layer of Ammon's horn, in the superficial layers of the entorhinal cortex and in the indusium griseum. Binding sites were visualized in all the subdivisions of the neocortex. The highest density of binding was generally detected in the outer edge of the superficial layer I. In some cortical areas, especially in the visual cortex, labeling with a prevalent laminar distribution in the superficial layers I-III and in the deep layers V-VI was clearly observed. Moderate to low densities of binding sites were seen in most other areas of the brain except in the white matter, the caudate nucleus and putamen, which were devoid of labeling.Overall these findings indicate a good correlation between the distribution of [H-3]nisoxetine binding sites and the noradrenergic systems. Furthermore, data suggest that in several areas, high-affinity noradrenaline reuptake mechanisms could play an important role in local interactions between the noradrenergic system and the other monoaminergic systems.