Pattern of expression of immune-relevant genes in the gonad of a teleost, the gilthead seabream (Sparus aurata L.)
Pattern of expression of immune-relevant genes in the gonad of a teleost, the gilthead seabream (Sparus aurata L.)
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DOI：
10.1016/j.molimm.2008.01.018
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发表时间：
2008-05-01
影响因子：
3.6
通讯作者：
Garcia-Ayala, A.
中科院分区：
文献类型：
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作者：
Chaves-Pozo, E.;Liarte, S.;Garcia-Ayala, A.
睾丸中的免疫反应受到调节，为发育中的男性生殖细胞提供保护，同时允许正常的炎症反应和防止感染。此外，生殖细胞是调节生殖过程的生长因子和细胞因子的有效靶点。本研究首次分析了季节性繁殖硬骨鱼生殖腺中几种免疫相关基因的表达模式。分析的免疫分子包括(i)炎症分子，如白细胞介素-1b （il1b）、il6、肿瘤坏死因子-a （tnfa）、环氧化酶-2 （cox2）和NADPH氧化酶亚基p40（phox）、ncf4基因；（ii）抗炎细胞因子转化生长因子b1 （tgfb1）及其2型受体tgfbr2；（iii）先天免疫受体，包括toll样受体9 （tlr9）、tlr5、tlr22和巨噬细胞集落刺激因子受体（mcsfr）；淋巴细胞受体，如t细胞受体P亚基（Tcrb）和免疫球蛋白M重链（ighm）；(v)抗菌分子溶菌酶（lyz）、hepcidin （hamp）和补体成分3 (c3)；（六）抗病毒分子粘病毒（流感）抗性蛋白（mx）；（vii）与白细胞浸润相关的分子，包括CC趋化因子ccl4、CXC趋化因子il8和白细胞粘附分子e -选择素（Sele）。值得注意的是，它们都显示出一种表达模式，这种表达模式取决于鱼作为雄性发育和功能的前两个生殖周期的生殖阶段。此外，我们证明了其中一些免疫相关分子，如Illb和Mcsfr，是由生殖细胞（Illb）和卵巢和睾丸体细胞（Mcsfr）产生的。这些数据表明，正如在哺乳动物中发生的那样，免疫分子之间存在着关键的平衡，这些平衡可能在鱼类配子发生的协调和性腺组织稳态的维持中发挥重要作用。(C) 2008 Elsevier Ltd版权所有。
Immune responses in the testis are regulated in a way that provides protection for the developing male germ cells, while permitting qualitatively normal inflammatory responses and protection against infection. In addition, germ cells are potent targets for the growth factors and cytokines which regulate the reproductive process. Our study analyzes for the first time the pattern of expression of several immune-relevant genes in the gonad of a seasonal breeding teleost fish. The immune molecules analyzed include (i) inflammatory molecules, such as interleukin-1b (il1b), il6, tumor necrosis factor-a (tnfa), cyclooxygenase-2 (cox2) and the NADPH oxidase subunit p40(phox), (ncf4 gene); (ii) the anti-inflammatory cytokine transforming growthfactor-b1 (tgfb1) and its type 2 receptor tgfbr2; (iii) innate immune receptors, including toll-like receptor 9 (tlr9), tlr5, tlr22 and macrophage-colony stimulating factor receptor (mcsfr); (iv) lymphocyte receptors, such as the P subunit of T-cell receptor (Tcrb) and the heavy chain of immunoglobulin M (ighm); (v) the anti-bacterial molecules lysozyme (lyz), hepcidin (hamp) and complement component 3 (c3); (vi) the anti-viral molecule myxovirus (influenza) resistance protein (mx); and (vii) molecules related to leukocyte infiltration, including the CC chemokine ccl4, the CXC chemokine il8 and the leukocyte adhesion molecule E-selectin (Sele). Notably, all of them show a pattern of expression that depends on the reproductive stage of the first two reproductive cycles when the fish develop and function as males. Furthermore, we demonstrate that some of these immune-relevant molecules, such as Illb and Mcsfr, are produced by germ cells (Illb) and ovarian and testicular somatic cells (Mcsfr). These data suggest that, as occurs in mammals, there is a critical balance between immune molecules and that these may play an essential role in the orchestration of gametogenesis and the maintenance of gonad tissue homeostasis in fish. (C) 2008 Elsevier Ltd. All rights reserved.