An oxaloacetate decarboxylase homologue protein influences the intracellular survival of Legionella pneumophila.
An oxaloacetate decarboxylase homologue protein influences the intracellular survival of Legionella pneumophila.
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DOI：
10.1111/j.1574-6968.1996.tb08589.x
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发表时间：
1996-12
影响因子：
2.1
通讯作者：
Bunty Jain;Bettina C. Brand;P. Lück;Marco Di Berardino;Peter Dimroth;Jörg Hacker
中科院分区：
文献类型：
--
作者：
Bunty Jain;Bettina C. Brand;P. Lück;Marco Di Berardino;Peter Dimroth;Jörg Hacker
嗜肺军团菌是一种兼性细胞内寄生虫，能够在各种真核细胞中存活。我们鉴定了嗜肺乳杆菌Corby菌株的tn5突变体，并能够确定转座子的插入位点。它位于一个开放阅读框内，与肺炎克雷伯菌草酰乙酸脱羧酶（OadA）的α -亚基高度同源。Western blotting检测嗜肺乳杆菌野生型菌株OadA同源蛋白。由于oadA突变菌株在豚鼠肺泡巨噬细胞和人单核细胞中的细胞内增殖减少，因此可以推断oadA基因产物对嗜肺乳杆菌的细胞内存活有影响。
Legionella pneumophila is a facultative intracellular parasite which is able to survive in various eukaryotic cells. We characterised a Tn5-mutant of the L. pneumophila Corby strain and were able to identify the insertion site of the transposon. It is localised within an open reading frame which shows high homology to the alpha-subunit of the oxaloacetate decarboxylase (OadA) of Klebsiella pneumoniae. The OadA homologous protein of L. pneumophila was detected in the wild-type strain by Western blotting. Since the intracellular multiplication of the oadA- mutant strain is reduced in guinea pig alveolar macrophages and human monocytes, it is concluded that the oadA gene product has an effect on the intracellular survival of L. pneumophila.