PLIN3 is up-regulated and correlates with poor prognosis in clear cell renal cell carcinoma
PLIN3 is up-regulated and correlates with poor prognosis in clear cell renal cell carcinoma
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PLIN3 上调并与透明细胞肾细胞癌的不良预后相关
DOI：
10.1016/j.urolonc.2018.04.006
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发表时间：
2018-07-01
影响因子：
2.7
通讯作者：
Zhang, XiaoPing
中科院分区：
文献类型：
--
作者：
Wang, Keshan;Ruan, HaiLong;Zhang, XiaoPing
背景：PLIN 3是围脂蛋白家族成员之一，参与脂滴的形成和积聚。方法：采用生物信息学方法分析PLIN 3在肾细胞癌（renal cell carcinoma，RCC）中的表达水平及其与临床病理参数的关系。通过蛋白质印迹、免疫荧光和免疫组织化学测定来确定PLIN 3在人RCC组织和细胞系中的表达水平。结果：PLIN 3在肾癌组织和细胞系中表达水平均升高，与TCGA和Oncomine肿瘤数据库分析结果一致。受试者工作特征曲线表明，PLIN 3高表达可以区分癌组织和正常肾组织（曲线下面积= 0.7270，P < 0.0001）。Kaplan-Meier曲线显示，PLIN 3的高表达预示着较低的无病生存率和总生存率。结论：PLIN 3在肾癌组织中高表达，PLIN 3的高表达可作为肾癌诊断和预后的生物学指标。PLIN 3功能抑制可作为一种新的临床治疗选择。(C)2018爱思唯尔公司All rights reserved.
Background: PLIN3, one of the members of the perilipin family, has been reported to be involved in the formation and accumulation of lipid droplets. However, the expression levels and diagnostic and prognostic value of PLIN3 in renal cell carcinoma (RCC) remain unclear.Methods: Bioinformatic analysis was used to assess the levels of PLIN3 and the correlation between PLIN3 levels and clinicopathological parameters in renal cancer. The expression levels of PLIN3 were determined in human RCC tissues and cell lines by western blot, immunofluorescence and immunohistochemistry assays. Receiver operating characteristic curves and Kaplan-Meier curves were used to analyze the diagnostic and prognostic significance of PLIN3 in RCC.Results: The expression level of PLIN3 was elevated in RCC tissues and cell lines, which was consistent with the analysis of the TCGA and Oncomine cancer database. The receiver operating characteristic curve indicated that high PLIN3 expression can distinguish cancer tissues from normal kidney tissues (area under the curve = 0.7270, P < 0.0001). Kaplan-Meier curves revealed that elevated PLIN3 predicted poor disease-free survival and overall survival.Conclusions: PLIN3 is highly expressed in kidney cancer, and high expression of PLIN3 can serve as a useful diagnostic and prognostic biomarker. PLIN3 functional inhibition can be used as a new clinical treatment option. (C) 2018 Elsevier Inc. All rights reserved.