CONDITIONING OF PARSLEY (PETROSELINUM-CRISPUM L) SUSPENSION CELLS INCREASES ELICITOR-INDUCED INCORPORATION OF CELL-WALL PHENOLICS
CONDITIONING OF PARSLEY (PETROSELINUM-CRISPUM L) SUSPENSION CELLS INCREASES ELICITOR-INDUCED INCORPORATION OF CELL-WALL PHENOLICS
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DOI：
10.1104/pp.102.2.459
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发表时间：
1993-06-01
期刊：
影响因子：
7.4
通讯作者：
MATERN, U
中科院分区：
文献类型：
--
作者：
KAUSS, H;FRANKE, R;MATERN, U
研究了诱导子诱导欧芹（Petroselinum crispum L.）悬浮培养物可以通过用2，6-二氯异烟酸或水杨酸衍生物预处理培养物来增强。为了进一步研究这一现象，从对照细胞或用来自Phytophthora megasperma f.大豆根瘤菌碱水解后，从引发的细胞的两个馏分显示出大大增加的4-香豆酸，阿魏酸，和4-羟基苯甲酸，以及4-羟基苯甲醛和香草醛的含量。两个次要峰被鉴定为酪醇和甲氧基酪醇。预处理效果在低激发子浓度下最为显著。其特异性阐述了香豆素分泌。当欧芹悬浮培养物与2，6-二氯异烟酸，4-或5-氯水杨酸，或3，5-二氯水杨酸预孵育1 d时，细胞表现出大大增加的激发子响应。用异烟酸、水杨酸、乙酰水杨酸或2，6-二羟基苯甲酸预处理在增强反应方面效率较低，并且一些其他异构体无活性。对于上述单体酚类化合物，也观察到激发子响应的增加，所述单体酚类化合物在碱水解时从细胞壁释放，并且对于通过巯基乙酸方法测定的“木质素样”细胞壁聚合物也观察到激发子响应的增加。对于5-氯水杨酸显示，调节最有可能改善信号转导，导致编码苯丙氨酸解氨酶和4-香豆酸：辅酶A连接酶的基因的激活。因此，调节使欧芹悬浮细胞对较低的激发子浓度敏感。如果将类似的机制应用于用2，6-二氯异烟酸（一种已知的系统获得性抗性诱导剂）处理的整株植物，则可以假设真菌病原体可能更容易和有效地被识别。
The elicitor-induced incorporation of phenylpropanoid derivatives into the cell wall and the secretion of soluble coumarin derivatives (phytoalexins) by parsley (Petroselinum crispum L.) suspension cultures can be potentiated by pretreatment of the cultures with 2,6-dichloroisonicotinic acid or derivatives of salicylic acid. To investigate this phenomenon further, the cell walls and an extracellular soluble polymer were isolated from control cells or cells treated with an elicitor from Phytophthora megasperma f. sp. glycinea. After alkaline hydrolysis, both fractions from elicited cells showed a greatly increased content of 4-coumaric, ferulic, and 4-hydroxybenzoic acid, as well as 4-hydroxybenzaldehyde and vanillin. Two minor peaks were identified as tyrosol and methoxytyrosol. The pretreatment effect is most pronounced at a low elicitor concentration. Its specificity was elaborated for coumarin secretion. When the parsley suspension cultures were preincubated for 1 d with 2,6-dichloroisonicotinic, 4- or 5-chlorosalicylic, or 3,5-dichlorosalicylic acid, the cells exhibited a greatly increased elicitor response. Pretreatment with isonicotinic, salicylic, acetylsalicylic, or 2,6-dihydroxybenzoic acid was less efficient in enhancing the response, and some other isomers were inactive. This increase in elicitor response was also observed for the above-mentioned monomeric phenolics, which were liberated from cell walls upon alkaline hydrolysis and for ''lignin-like' cell wall polymers determined by the thioglycolic acid method. It was shown for 5-chlorosalicylic acid that conditioning most likely improves the signal transduction leading to the activation of genes encoding phenylalanine ammonia lyase and 4-coumarate: coenzyme A ligase. The conditioning thus sensitizes the parsley suspension cells to respond to lower elicitor concentrations. If a similar mechanism were to apply to whole plants treated with 2,6-dichloroisonicotinic acid, a known inducer of systemic acquired resistance, one can hypothesize that fungal pathogens might be recognized more readily and effectively.