Preliminary Comparison of Endoscopic Brush and Net Catheters as the Sampling Tool to Analyze the Intestinal Mucus in the Rectum with Ulcerative Colitis Patients
Preliminary Comparison of Endoscopic Brush and Net Catheters as the Sampling Tool to Analyze the Intestinal Mucus in the Rectum with Ulcerative Colitis Patients
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DOI：
10.1159/000521929
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发表时间：
2022-02-18
期刊：
影响因子：
3.2
通讯作者：
Kawashima, Hiroki
中科院分区：
文献类型：
--
作者：
Nakamura, Masanao;Maeda, Keiko;Kawashima, Hiroki
背景：溃疡性结肠炎（UC）的病理生理尚不清楚，但结直肠粘膜表面的早期病变可能在其病因中起重要作用。肠道粘液样本，包括内层和外层，是通过网或刷导管收集的，但每种方法获得的样本质量尚未得到充分的调查。目的：比较结肠镜检查时用网状导管和刷状导管收集的肠道粘液的微生物组和蛋白质含量。方法：对4例UC患者采用网管、刷管、肠液吸取下直肠肠黏液标本。采用16S rRNA基因测序和质谱分析微生物组和蛋白质含量。结果：两组患者的α微生物组多样性差异有统计学意义（p < 0.05），但两种取样方法间无差异。净导管样品的总蛋白浓度高于刷导管样品。与净组相比，刷导管组有更多的毛螺旋体，一种产生丁酸盐的细菌。与净组相比，刷管组在活跃期的口腔中葡萄球菌和Dialister的细菌也更多。结论：刷状导管更容易收集肠黏液内层，而净状导管更容易收集包括外黏液层和肠液在内的较大样本。两种采样方法，不同类型的粘膜采集可能导致不同的结果在粘膜易感性患者。
Background: The pathophysiology of ulcerative colitis (UC) remains unclear, but early lesions on the colorectal mucosal surface may play an important role in its etiology. Intestinal mucus samples, including inner and outer layers, are collected by net or brush catheters, but the quality of the samples obtained by each method has not been fully investigated. Objective: The purpose of this study was to compare the microbiome and protein content of intestinal mucus collected by net and brush catheters during colonoscopy. Methods: Intestinal mucus samples from the lower rectum of 4 patients with UC were collected using a net catheter, a brush catheter, and intestinal fluid suction. Microbiome and protein content were analyzed using 16S rRNA gene sequencing and mass spectrometry. Results: The patients demonstrated significant differences in microbiome alpha diversity (p < 0.05), but this difference was not observed between the sampling methods. Net catheter samples demonstrated higher total protein concentrations than brush catheter samples. The brush catheter group had more Lachnospira, a butyrate-producing bacterium, when compared to the net group. The brush catheter group also had more oral bacteria of Staphylococcus and Dialister in those with active phase when compared to the net group. Conclusions: Brush catheters are more likely to collect the intestinal mucus inner layer, whereas net catheters are more likely to collect larger samples that include the outer mucus layer, as well as the intestinal fluid. Two sampling methods with different types of collection of the mucosa may lead to different results among patients with mucosal vulnerabilities.