Wingless repression of Drosophila frizzled 2 expression shapes the wingless morphogen gradient in the wing
Wingless repression of Drosophila frizzled 2 expression shapes the wingless morphogen gradient in the wing
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DOI：
10.1016/s0092-8674(00)81438-5
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发表时间：
1998-05-29
期刊：
影响因子：
64.5
通讯作者：
Nusse, R
中科院分区：
文献类型：
--
作者：
Cadigan, KM;Fish, MP;Nusse, R
在果蝇翅成虫盘中，Wingless（Wg）蛋白作为形态发生蛋白，从盘的背/腹（D/V）边界发出，以在短距离和长距离上沿沿着D/V轴直接定义细胞身份。在这里，我们表明，高水平的Wg受体，果蝇frizzled 2（Dfz 2），稳定Wg，使其能够到达细胞远离其合成的网站。Wg信号传导抑制Dfz 2表达，产生朝向D/V边界移动的Wg稳定性降低的梯度。Dfz 2的这种抑制对于Wg形态原梯度的正常形状以及细胞对Wg信号的响应至关重要。与受体限制配体扩散的其他配体-受体关系相反，Dfz 2通过保护Wg免于降解来扩大Wg作用的范围。
In Drosophila wing imaginal discs, the Wingless (Wg) protein acts as a morphogen, emanating from the dorsal/ventral (D/V) boundary of the disc to directly define cell identities along the D/V axis at short and long range. Here, we show that high levels of a Wg receptor, Drosophila frizzled 2 (Dfz2), stabilize Wg, allowing it to reach cells far from its site of synthesis. Wg signaling represses Dfz2 expression, creating a gradient of decreasing Wg stability moving toward the D/V boundary. This repression of Dfz2 is crucial for the normal shape of Wg morphogen gradient as well as the response of cells to the Wg signal. In contrast to other ligand-receptor relationships where the receptor limits diffusion of the ligand, Dfz2 broadens the range of Wg action by protecting it from degradation.