Induction of apoptosis by an inhibitor of EGFR in neuroblastoma cells
Induction of apoptosis by an inhibitor of EGFR in neuroblastoma cells
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DOI：
10.1016/j.bbrc.2007.04.124
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发表时间：
2007-06-22
影响因子：
3.1
通讯作者：
Sugimoto, Tohru
中科院分区：
文献类型：
--
作者：
Tamura, Shinichi;Hosoi, Hajime;Sugimoto, Tohru
我们的目的是检测神经母细胞瘤组织中 EGFR 的表达，并研究选择性 EGFR-酪氨酸激酶抑制剂吉非替尼对神经母细胞瘤的抗肿瘤活性。通过免疫组织化学检测两种肿瘤组织中的EGFR表达，通过Western blotting检测10个细胞系中的8个细胞系中的EGFR表达。吉非替尼抑制 EGFR 磷酸化和体外细胞生长（IC50：约 1.2 μM），高浓度吉非替尼（20-30 μM）在体外诱导细胞凋亡。这是首次报道 EGFR 蛋白在神经母细胞瘤组织和细胞系的细胞表面表达。我们还证明了 EGFR 抑制剂可诱导神经母细胞瘤细胞凋亡。我们的结果表明靶向 EGFR 作为对抗神经母细胞瘤的新策略的可行性。 (c) 2007 Elsevier Inc. 保留所有权利。
We aimed to examine the expression of EGFR in neuroblastoma tissues and to investigate the antitumor activity of a selective EGFR-tyrosine kinase inhibitor, gefitinib, on neuroblastoma. The expression of EGFR was detected in each of two tumor tissues by immunohistochemistry and eight of 10 cell lines by Western blotting. Gefitinib inhibited EGFR-phosphorylation and in vitro cell growth (IC50: approximately 1.2 mu M), and a high concentration of gefitinib (20-30 mu M) induced apoptosis in vitro. This is the first report that EGFR protein is expressed on the cell surface in neuroblastoma tissues and in cell lines. We also demonstrated an EGFR inhibitor induced apoptosis on neuroblastoma, cells. Our results suggest the feasibility of targeting EGFR as a novel strategy against neuroblastoma. (c) 2007 Elsevier Inc. All rights reserved.