Impairment of Lhca4, a subunit of LHCI, causes high accumulation of chlorophyll and the stay-green phenotype in rice.
Impairment of Lhca4, a subunit of LHCI, causes high accumulation of chlorophyll and the stay-green phenotype in rice.
复制标题
DOI：
10.1093/jxb/erx468
复制
发表时间：
2018-02-23
影响因子：
6.9
通讯作者：
Kusaba M
中科院分区：
文献类型：
--
作者：
Yamatani H;Kohzuma K;Nakano M;Takami T;Kato Y;Hayashi Y;Monden Y;Okumoto Y;Abe T;Kumamaru T;Tanaka A;Sakamoto W;Kusaba M
LHCI亚基Lhca 4的缺失导致水稻叶绿素含量和LHC II积累增加，这表明LHCI和LHC II之间存在一种新的功能相互作用。叶绿素是光合作用中获取光能的重要分子。在叶片衰老期间导致叶绿素保留的突变被称为“保绿”突变体。几种类型的持绿突变体之一，E型，在衰老前的叶片中积累高水平的叶绿素，导致延迟变黄。我们分离到了延迟黄化1 -1（dye 1 -1），这是一个在田间黄化延迟的水稻突变体。dye 1 -1在衰老前和衰老叶中积累了比野生型更多的叶绿素，但在“衰老绿色叶片”中没有保留叶功能，这表明dye 1 -1是E型保持绿色突变体。定位克隆显示，DYE 1编码Lhca 4，捕光复合物I（LHCI）的一个亚基。在染料1 -1中，氨基酸取代发生在参与色素结合的高度保守的氨基酸残基的位置处;事实上，在蓝色非变性PAGE分析中观察到染料1 -1中PSI-LHCI超复合物的严重受损的结构。尽管如此，生物量和碳同化率的染料1 -1是在野生型。有趣的是，Lhcb 1，一个三聚体LHCII蛋白，高度积累在染料1 -1，在叶绿素-蛋白质复合物。在LHCI突变染料中LHCII的高积累表明LHCI和LHCII之间的新的功能相互作用。
Impairment of Lhca4, a subunit of LHCI, causes high chlorophyll content and LHCII accumulation in rice, suggesting a novel functional interaction between LHCI and LHCII. Chlorophyll is an essential molecule for acquiring light energy during photosynthesis. Mutations that result in chlorophyll retention during leaf senescence are called ‘stay-green’ mutants. One of the several types of stay-green mutants, Type E, accumulates high levels of chlorophyll in the pre-senescent leaves, resulting in delayed yellowing. We isolated delayed yellowing1-1 (dye1-1), a rice mutant whose yellowing is delayed in the field. dye1-1 accumulated more chlorophyll than the wild-type in the pre-senescent and senescent leaves, but did not retain leaf functionality in the ‘senescent green leaves’, suggesting that dye1-1 is a Type E stay-green mutant. Positional cloning revealed that DYE1 encodes Lhca4, a subunit of the light-harvesting complex I (LHCI). In dye1-1, amino acid substitution occurs at the location of a highly conserved amino acid residue involved in pigment binding; indeed, a severely impaired structure of the PSI-LHCI super-complex in dye1-1 was observed in a blue native PAGE analysis. Nevertheless, the biomass and carbon assimilation rate of dye1-1 were comparable to those in the wild-type. Interestingly, Lhcb1, a trimeric LHCII protein, was highly accumulated in dye1-1, in the chlorophyll–protein complexes. The high accumulation of LHCII in the LHCI mutant dye1 suggests a novel functional interaction between LHCI and LHCII.
登录
查看更多内容
影响因子：
7.2
作者：
Morita, Ryouhei;Sato, Yutaka;Kusaba, Makoto
通讯作者：
Kusaba, Makoto
DOI：
10.1073/pnas.0605421103
发表时间：
2006-11-21
影响因子：
11.1
作者：
Naito, Ken;Cho, Eunyoung;Wessler, Susan R.
通讯作者：
Wessler, Susan R.
影响因子：
11.6
作者：
Pesaresi, Paolo;Hertle, Alexander;Leister, Dario
通讯作者：
Leister, Dario
影响因子：
7.4
作者：
Ganeteg, U;Külheim, C;Jansson, S
通讯作者：
Jansson, S
影响因子：
64.8
作者：
Lunde, C;Jensen, PE;Scheller, HV
通讯作者：
Scheller, HV