Patterning of avian craniofacial muscles.
Patterning of avian craniofacial muscles.
复制标题
鸟类颅面肌肉的图案。
DOI：
10.1016/0012-1606(86)90138-7
复制
发表时间：
1986
影响因子：
2.7
通讯作者：
Noden,DM
中科院分区：
文献类型：
--
作者：
Noden,DM
脊椎动物的随意肌由肌管和结缔组织细胞组成。这两种细胞类型有不同的胚胎起源：肌源性细胞来自近轴中胚层，而在头部，许多结缔组织由神经嵴细胞形成。本研究的目的是研究异位移植的躯干肌节细胞和假定的结缔组织形成的头神经嵴间充质之间的相互作用。假定或新形成的颈部体节从鹌鹑胚胎植入侧鸡主机的中脑神经嵴迁移发病前。在孵育7至12天之间处死鹌鹑，并检查切片中是否存在鹌鹑细胞。一些移植组织在原位分化，形成异位骨骼、结缔和肌肉组织。然而，许多肌瘤细胞脱离植入物，成为整合到相邻的神经嵴间充质，并随后形成正常的外部眼或颌肌肉。在这些肌肉中，很明显，只有肌源性群体来自移植的干细胞。辅助的发现是，移植的躯干近轴中胚层经常干扰神经嵴细胞，形成角膜后上皮和基质组织的运动，和一些移植的细胞形成异位膜内骨邻近的眼睛。这些结果证实，假定结缔组织形成间充质来源于神经嵴赋予空间图案的信息后，肌原细胞侵入it.Further，肌瘤细胞和侧板体细胞中胚层在躯干和神经嵴间充质在头部之间的相互作用似乎根据类似的机制。
Vertebrate voluntary muscles are composed of myotubes and connective tissue cells. These two cell types have different embryonic origins: myogenic cells arise from paraxial mesoderm, while in the head many of the connective tissues are formed by neural crest cells. The objective of this research was to study interactions between heterotopically transplanted trunk myotomal cells and presumptive connective tissue-forming cephalic neural crest mesenchyme. Presumptive or newly formed cervical somites from quail embryos were implanted lateral to the midbrain of chick hosts prior to the onset of neural crest emigration. Hosts were sacrificed between 7 and 12 days of incubation, and sections examined for the presence of quail cells. Some grafted tissues differentiatedin situ, forming ectopic skeletal, connective, and muscle tissues. However, many myotomal cells broke away from the implant, became integrated into adjacent neural crest mesenchyme, and subsequently formed normal extrinsic ocular or jaw muscles. In these muscles it was evident that only the myogenic populations were derived from grafted trunk cells. Ancillary findings were that grafted trunk paraxial mesoderm frequently interfered with the movement of neural crest cells which form the corneal posterior epithelial and stromal tissues, and that some grafted cells formed ectopic intramembranous bones adjacent to the eye. These results verify that presumptive connective tissue-forming mesenchyme derived from the neural crest imparts spatial patterning information upon myogenic cells that invade it. Moreover, interactions between myotomal cells and both lateral plate somatic mesoderm in the trunk and neural crest mesenchyme in the head appear to operate according to similar mechanisms.