Effects of Krill-derived phospholipid-enriched n-3 fatty acids on Ca(2+) regulation system in cerebral arteries from ovariectomized rats
Effects of Krill-derived phospholipid-enriched n-3 fatty acids on Ca(2+) regulation system in cerebral arteries from ovariectomized rats
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磷虾磷脂n-3脂肪酸对去势大鼠脑动脉Ca(2)调节系统的影响
DOI：
10.1016/j.lfs.2014.01.070
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发表时间：
2014
期刊：
影响因子：
6.1
通讯作者：
Kenichi Yanagimoto
中科院分区：
文献类型：
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作者：
Yasushi Sakai;Michio Hashimoto;Budbazar Enkhjargal;Hisashi Mitsuishi;Hiromi Nobe;Ichiro Horie;Takahiro Iwamoto;Kenichi Yanagimoto
目的为探讨n − 3多不饱和脂肪酸对脑循环的影响，给去卵巢（OVX）大鼠灌胃磷虾油中的磷脂（KPL）或鱼油中的甘油三酯（FTG），然后分析其对基底动脉（BA）Ca 2+调节系统的影响。主要方法大鼠分为4组：对照、OVX、给予KPL的OVX（OVXP）和给予FTG的OVX（OVXT），每天口服，持续2周。结果：① ①在对照组中，5-HT引起的时间依赖性舒张反应（TDR）明显降低; ②在OVXP和OVXT中，TEA对TDR有部分抑制作用，而在对照组中，TEA对TDR有部分抑制作用。在对照组、OVXP和OVXT中，TEA可抑制5-HT预收缩的BA中加入5 mM KCl诱导的舒张，但在OVX中不抑制。FTG能显著抑制去卵巢大鼠脑内NCX 1 mRNA的过表达。KPL和FTG能显著抑制去卵巢大鼠脑动脉ADP/ATP比值。卵巢功能不全影响Ca ~（2+）激活的、ATP敏感的K ~+通道和在脑血流自动调节中起重要作用的NCX 1。此外，KPL可能成为绝经后妇女的FTG一样好的补充。
AimsTo investigate the effects of n − 3 polyunsaturated fatty acids on cerebral circulation, ovariectomized (OVX) rats were administered with phospholipids in krill oil (KPL) or triglycerides in fish oil (FTG); effects on the Ca2 +regulating system in their basilar artery (BA) were then analyzed.Main methodsThe rats were divided into 4 groups: control, OVX, OVX given KPL (OVXP), and OVX given FTG (OVXT) orally, daily for 2 weeks. Time dependent relaxation (TDR) of contractile response to 5HT in BA was determined myographically, Na+/Ca2 +exchanger (NCX) 1 mRNA expression was determined by real time PCR, and nucleotides were analyzed by HPLC.Key findingsThe level of TDR in OVX that was significantly lower in the control was inhibited byl-NAME and indomethacin; TEA inhibited TDR totally in the control but only partly in OVXP and OVXT. Relaxation induced by the addition of 5 mM KCl to the BA pre-contracted with 5-HT was inhibited by TEA in the controls, OVXP and OVXT, but not in OVX. Overexpression of NCX1 mRNA in the BA from OVX was significantly inhibited by FTG. The ratio of ADP/ATP in cerebral arteries from OVX was significantly inhibited by KPL and FTG. Levels of triglyceride and arachidonic acid in the plasma of OVX increased, but were significantly inhibited by KPL and FTG.SignificanceOvarian dysfunction affects Ca2 +activated-, ATP-sensitive-K+channels and NCX1, which play crucial roles in the autoregulation of cerebral blood flow. Also, KPL may become as good a supplement as FTG for postmenopausal women.