SEPARATION OF DNA-BINDING FROM THE TRANSCRIPTION-ACTIVATING FUNCTION OF A EUKARYOTIC REGULATORY PROTEIN
SEPARATION OF DNA-BINDING FROM THE TRANSCRIPTION-ACTIVATING FUNCTION OF A EUKARYOTIC REGULATORY PROTEIN
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DOI：
10.1126/science.3080805
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发表时间：
1986-02-14
期刊：
影响因子：
56.9
通讯作者：
PTASHNE, M
中科院分区：
文献类型：
--
作者：
KEEGAN, L;GILL, G;PTASHNE, M
酵母GAL4蛋白(881个氨基酸)与目标基因上游的特定DNA位点结合并激活转录。这种蛋白质的衍生物只有74个氨基酸末端残基，可以与这些位点结合，但不能激活转录。当这些衍生物被适当地定位在基因前面时，它们就起到了抑制因子的作用。这些和相关的发现支持这样的观点，即GAL4通过接触其他DNA结合蛋白来激活转录。
The yeast GAL4 protein (881 amino acids) binds to specific DNA sites upstreams of target genes and activates transcription. Derivatives of this protein bearing as few as 74 amino terminal residues bind to these sites but fail to activate transcription. When appropriately positioned in front of a gene these derivatives act as repressors. These and related findings support the idea that GAL4 activates transcription by touching other DNA-bound proteins.