High-throughput synthesis of stable isotope-labeled transmembrane proteins for targeted transmembrane proteomics using a wheat germ cell-free protein synthesis system
High-throughput synthesis of stable isotope-labeled transmembrane proteins for targeted transmembrane proteomics using a wheat germ cell-free protein synthesis system
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DOI：
10.1039/c4mb00556b
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发表时间：
2015-01-01
影响因子：
--
通讯作者：
Higashiyama, Shigeki
中科院分区：
文献类型：
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作者：
Takemori, Nobuaki;Takemori, Ayako;Higashiyama, Shigeki
利用小麦无胚细胞蛋白质合成系统，我们开发了一种高通量合成稳定同位素标记的全长跨膜蛋白作为蛋白脂质体的方法，以模拟体内环境，我们成功地构建了一个内标文库靶向跨膜蛋白质组学使用质谱。
Using a wheat germcell-free protein synthesis system, we developed a high-throughput method for the synthesis of stable isotope-labeled full-length transmembrane proteins as proteoliposomes to mimic the in vivo environment, and we successfully constructed an internal standard library for targeted transmembrane proteomics by using mass spectrometry.