Divisio of Mitochondrial Nuclei in Protozoa, a Green Alga and a Higher Plant
Divisio of Mitochondrial Nuclei in Protozoa, a Green Alga and a Higher Plant
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原生动物、绿藻和高等植物线粒体核的分裂
DOI：
10.1508/cytologia.50.75
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发表时间：
1985
期刊：
影响因子：
1
通讯作者：
T. Kuroiwa
中科院分区：
文献类型：
--
作者：
S. Nishibayashi;T. Kuroiwa
用4′-6-二氨基-2-苯基吲哚（DAPI）荧光显微镜研究了变形变形虫、尾草履虫、柔韧性Nitella flexilis和洋葱（Allium cepa）线粒体核的分裂。变形变形虫和尾草履虫的线粒体各含1个mt-核，弹性Nitella flexilis和葱属Allium cepa的线粒体各含1 - 2个mt-核。多头绒泡菌、变形变形虫、尾草履虫、柔韧Nitella flexilis和洋葱的一个T4噬菌体DNA分子与球形线粒体mt-核的荧光强度（负膜颗粒密度）之比约为1:16:22:5:0.4:0.4。即弹性Nitella flexilis和葱属Allium cepa的线粒体DNA含量远低于变形阿米巴变形虫和尾草履虫。变形变形虫、尾草履虫和多头绒泡菌的线粒体和细胞核是通过收缩同时分裂的，而弹性Nitella flexilis和葱属Allium cepa的线粒体分裂发生在线粒体分裂之前。尾草草履虫和葱属植物哑铃形线粒体的mt核荧光强度约为球形线粒体的2倍。因此，线粒体DNA可能会复制并平均分成子线粒体。
The division of mitochnodrial nuclei in Amoeba proteus, Paramecium caudatum, Nitella flexilis and Allium cepa was investigated using 4'-6-diamidino-2-phenylindole (DAPI) epifluorescent microscopy. The mitochondria each contained one mt-nucleus in Amoeba proteus and Paramecium caudatum and one or two mt-nuclei in Nitella flexilis and Allium cepa. The ratio of fluorescent intensity (grain density of negative film) among one DNA molecule of a T4 phage particle and mt-nuclei of spherical mitochondria in Physarum polycepaalum, Amoeba proteus, Paramecium caudatum, Nitella flexilis and Allium cepa was about 1:16:22:5:0.4:0.4. That is, the mitochondrial DNA content in Nitella flexilis and Allium cepa was much smaller than those in Amoeba proteus and Paramecium caudatum. The mitochondria and their nuclei in Amoeba proteus and Paramecium caudatum as well as Physarum polycephalum divided simultaneously by constriction, while segregation of mitochondria) nuclei occurred before the mitochondrial division in Nitella flexilis and Allium cepa. The fluorescent intensity of mt-nuclei in dumbbell-shaped mitochondria of Paramecium caudatum and Allium cepa was about twice that in spherical mitochondria. Therefore, mitochondrial DNA probably duplicated and divided equally into daughter mitochondria.