A method to measure the rate of net fluid secretion by monolayers of cultured renal epithelial cells

A method to measure the rate of net fluid secretion by monolayers of cultured renal epithelial cells
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测量单层培养肾上皮细胞净液体分泌率的方法

DOI:
10.1007/bf02388131
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发表时间:
1991
期刊:
Journal of Tissue Culture Methods
影响因子:
--
通讯作者:
J. Grantham
J. Grantham
中科院分区:
--
文献类型:
--
作者:
T. K. Neufeld;M. Grant;J. Grantham

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我们描述了一种明确且高度可重复的方法来测量培养的肾上皮单层的净液体分泌量。该方法还可用于测量分泌液的成分以及净溶质和液体运输的激动剂和抑制剂的作用。
We describe an unambiguous and highly reproducible method to measure net fluid secretion across cultured renal epithelial monolayers. The method can also be used to measure the constituents of secreted fluid and the effect of agonists and inhibitors of net solute and fluid transport.
DOI: 10.1073/pnas.86.15.6007
发表时间: 1989-08-01
影响因子: 11.1
作者:
MANGOOKARIM, R;UCHIC, M;GRANTHAM, JJ
通讯作者: GRANTHAM, JJ