Enhancement of fatty acyl-CoA oxidizing activity in rat liver peroxisomes by di-(i-ethylhexyl)phthalate.
Enhancement of fatty acyl-CoA oxidizing activity in rat liver peroxisomes by di-(i-ethylhexyl)phthalate.
复制标题
邻苯二甲酸二(异乙基己基)酯增强大鼠肝脏过氧化物酶体中脂肪酰辅酶A的氧化活性。
DOI：
10.1093/oxfordjournals.jbchem.a132044
复制
发表时间：
1978
影响因子：
2.7
通讯作者：
T. Hashimoto
中科院分区：
文献类型：
--
作者：
T. Osumi;T. Hashimoto
研究了邻苯二甲酸二(2-乙基己基)酯 (DEHP) 对肝脏中氰化物不敏感的棕榈酰辅酶 A 氧化活性的影响。两周的 DEHP 治疗使雄性 Wistar 大鼠的活性增加了一个数量级。在雄性和雌性斯普拉格-道利大鼠和小鼠中也观察到了类似的效果，但在豚鼠中没有观察到。当通过差速离心分离肝脏时，活性集中在轻质线粒体部分。通过蔗糖密度梯度离心对该级分进行亚分级，其活性分布模式与尿酸氧化酶相似，但与谷氨酸脱氢酶不同。这些数据表明，位于过氧化物酶体中的脂肪酰辅酶A氧化酶系统是由DEHP的施用诱导的。
The effect of di-(2-ethylhexyl)phthalate (DEHP) administration on cyanide-insensitive palmitoyl-CoA oxidizing activity in liver was studied. Two weeks of DEHP treatment increased the activity by one order of magnitude in male Wistar rats. A similar effect was also observed in male and female Sprague-Dawley rats and mice, but not in guinea pigs. When the liver was fractionated by differential centrifugation, the activity was concentrated in the light mitochondrial fraction. On the subfractionation of this fraction by sucrose density gradient centrifugation, the activity was distributed in a pattern similar to that of urate oxidase, but not resembling that of glutamate dehydrogenase. These data suggest that a fatty acyl-CoA oxidizing enzyme system which is located in peroxisomes is induced by the administration of DEHP.