Dengue emergence and adaptation to peridomestic mosquitoes
Dengue emergence and adaptation to peridomestic mosquitoes
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DOI：
10.3201/eid1010.030846
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发表时间：
2004-10-01
影响因子：
11.8
通讯作者：
Weaver, SC
中科院分区：
文献类型：
--
作者：
Moncayo, AC;Fernandez, Z;Weaver, SC
系统发育证据表明，在人类中由嗜人蚊子埃及伊蚊和白纹伊蚊传播的地方性和流行性登革热病毒（DENV），是当由野生伊蚊在非人灵长类动物中传播的祖先野生型DENV适应这些半家栖媒介时出现的。我们通过回顾性研究流行性和地方性登革热病毒2型毒株与野生毒株对白纹伊蚊和埃及伊蚊的适应性证据来检验这一假设。对来自美洲和东南亚不同地理区域的蚊子的第一代和第二代后代进行了测试，以确定它们对来自西非、东南亚和大洋洲的流行性/地方性和野生登革热病毒2型分离株的易感性。在吸食人工血餐后，这两种伊蚊对地方性/流行性登革热病毒2型毒株都高度易感（感染率高达100%），但对野生登革热病毒2型毒株的易感性明显较低（低至0%）。我们的研究结果支持这样的假设：对半家栖蚊子媒介的适应介导了登革热从野生祖病毒的出现。
Phylogenetic evidence suggests that endemic and epidemic dengue viruses (DENV), transmitted among humans by the anthropophilic mosquitoes Aedes aegypti and Ae. albopictus, emerged when ancestral, sylvatic DENV transmitted among nonhuman primates by sylvatic Aedes mosquitoes adapted to these peridomestic vectors. We tested this hypothesis by retrospectively examining evidence for adaptation of epidemic and endemic versus sylvatic strains of DENV-2 to Ae. albopictus and Ae. aegypti. First and second-generation offspring of mosquitoes from different geographic regions in the Americas and Southeast Asia were tested for their susceptibility to epidemic/endemic and sylvatic DENV-2 isolates from West Africa, Southeast Asia, and Oceania. Both Aedes species were highly susceptible (up to 100% infected) to endemic/epidemic DENV-2 strains after ingesting artificial blood meals but significantly less susceptible (as low as 0%) to sylvatic DENV-2 strains. Our findings support the hypothesis that adaptation to peridomestic mosquito vectors mediated dengue emergence from sylvatic progenitor viruses.