The ribosome receptors Mrx15 and Mba1 jointly organize cotranslational insertion and protein biogenesis in mitochondria
The ribosome receptors Mrx15 and Mba1 jointly organize cotranslational insertion and protein biogenesis in mitochondria
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DOI：
10.1091/mbc.e18-04-0227
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发表时间：
2018-10-01
影响因子：
3.3
通讯作者：
Ott, Martin
中科院分区：
文献类型：
--
作者：
Moller-Hergt, Braulio Vargas;Carlstrom, Andreas;Ott, Martin
酿酒酵母中的线粒体基因表达负责氧化磷酸化系统的高度疏水性亚基的产生。膜插入以共翻译方式发生在膜结合的线粒体核糖体上。在这里，通过采用基于系统质谱的方法，我们发现了以前未表征的膜蛋白 Mrx15，它通过可溶性 C 末端结构域与大核糖体亚基相互作用。 Mrx15 在合成过程中接触线粒体翻译产物，并与核糖体受体 Mba1 一起在共翻译蛋白插入中发挥重叠作用。总而言之，我们的数据揭示了这些核糖体受体如何组织线粒体中的膜蛋白生物发生。
Mitochondrial gene expression in Saccharomyces cerevisiae is responsible for the production of highly hydrophobic subunits of the oxidative phosphorylation system. Membrane insertion occurs cotranslationally on membrane-bound mitochondrial ribosomes. Here, by employing a systematic mass spectrometry-based approach, we discovered the previously uncharacterized membrane protein Mrx15 that interacts via a soluble C-terminal domain with the large ribosomal subunit. Mrx15 contacts mitochondrial translation products during their synthesis and plays, together with the ribosome receptor Mba1, an overlapping role in cotranslational protein insertion. Taken together, our data reveal how these ribosome receptors organize membrane protein biogenesis in mitochondria.