Long Non-coding RNA LINC01503 Promotes Gastric Cancer Cell Proliferation and Invasion by Regulating Wnt Signaling
Long Non-coding RNA LINC01503 Promotes Gastric Cancer Cell Proliferation and Invasion by Regulating Wnt Signaling
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DOI：
10.1007/s10620-020-06215-4
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发表时间：
2020-03-23
影响因子：
3.1
通讯作者：
Zhu, Yong
中科院分区：
文献类型：
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作者：
Ding, Jian;Shi, Feng;Zhu, Yong
背景研究表明，长链非编码RNA的调控异常在肿瘤中起重要作用。LINC 01503是一种新发现的lncRNA，可促进多种肿瘤类型的发展。然而，LINC 01503在胃癌中的作用尚未见报道。目的探讨LINC 01503在胃癌发生发展中的作用及其分子生物学调控机制。方法采用qRT-PCR方法检测LINC 01503在胃癌组织和胃癌细胞株中的表达。Transwell实验和细胞计数实验检测细胞的侵袭和增殖能力。通过Western blot和qRT-PCR分析C-myc、细胞周期蛋白D1和β-连环蛋白的表达。结果LINC 01503在胃癌组织和细胞系中呈高表达，且与预后不良相关。LINC 01503的敲低抑制胃癌细胞的增殖和侵袭，而LINC 01503的过表达则表现出相反的趋势。沉默LINC 01503显著抑制c-myc、细胞周期蛋白D1和β-连环蛋白的表达。过表达β-catenin可恢复LINC 01503沉默对胃癌细胞增殖和转移的抑制作用。结论LINC 01503的高表达可能通过正性调节Wnt/beta-catenin通路促进胃癌的增殖和转移。
Background Previous studies have indicated that the dysregulation of long non-coding RNAs plays an important role in tumors. LINC01503 is a newly discovered lncRNA that promotes development of various tumor types. However, the function of LINC01503 in gastric cancer has not been reported yet. Aims To explore the function of LINC01503 in gastric cancer development and the underlying molecular biological regulatory mechanisms. Methods LINC01503 expression in tissues and cell lines of gastric cancer were determined through qRT-PCR. Transwell assay and cell number counting experiments were employed to detect the cell invasion and proliferation. C-myc, cyclin D1, and beta-catenin expressions were analyzed through Western blot and qRT-PCR. Results LINC01503 was highly expressed in gastric cancer tissues and cell lines, which was correlated with poor prognosis. Knockdown of LINC01503 suppressed gastric cancer cell proliferation and invasion, whereas overexpression of LINC01503 showed a reverse trend. Silencing LINC01503 significantly inhibited the expression of c-myc, cyclin D1, and beta-catenin. Overexpressing beta-catenin rescued the inhibitory effects, induced by LINC01503 silencing, on gastric cancer cell proliferation and metastasis. Conclusions This research reported that the elevated expression of LINC01503 could promote proliferation and metastasis of gastric cancer through positively regulating the Wnt/beta-catenin pathway.