DRG microstimulation evokes postural responses in awake, standing felines
DRG microstimulation evokes postural responses in awake, standing felines
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DRG 微刺激可引起清醒站立猫科动物的姿势反应
DOI：
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发表时间：
2019
影响因子：
4
通讯作者：
D. J. Weber
中科院分区：
文献类型：
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作者：
Kevin W King;William F Cusack;A. Nanivadekar;Christopher A. Ayers;M A Urbin;R. Gaunt;L. Fisher;D. J. Weber
Objective.我们以前已经证明，在背根神经节（DRG）的微刺激可以选择性地唤起活动的初级传入神经元在麻醉猫。本研究描述了重点描述安静站立期间醒着的猫背根神经节微刺激的姿势影响的实验结果。Approach.为了了解可以影响这些姿势变化的刺激参数，我们测量了地面反作用力（GRF）的变化，同时改变刺激位置和幅度。四只动物在L 6和L7 DRG长期植入穿透多通道微电极阵列。在每周的测试中，我们确定了以快速（80-120 m s-1）和中等（30-75 m s-1）传导速度招募初级传入神经元的电极通道，并选择一个通道在安静站立期间提供电流控制的双相刺激序列。主要结果。通过GRF的变化确定姿势反应，并根据其幅度和潜伏期进行表征。在DRG微刺激期间，动物没有表现出明显的痛苦或不适体征，这可能表明疼痛或对伤害性感觉的厌恶。在总共56周的时间里，13个电极通道诱发了行为反应，如通过GRF的显著变化所检测到的。刺激幅度调制这13个通道的GRF响应的幅度（p < 0.001）的范围内。不可能根据传导速度、募集阈值或电极所在的DRG等信息来预测电极是否会驱动行为反应。意义对低振幅（<40 µA）DRG微刺激的姿势反应的明显和可重复的影响支持该技术可能是神经损伤（如截肢）后恢复体感反馈的有效方法。
Objective. We have demonstrated previously that microstimulation in the dorsal root ganglia (DRG) can selectively evoke activity in primary afferent neurons in anesthetized cats. This study describes the results of experiments focused on characterizing the postural effects of DRG microstimulation in awake cats during quiet standing. Approach. To understand the parameters of stimulation that can affect these postural shifts, we measured changes in ground reaction forces (GRF) while varying stimulation location and amplitude. Four animals were chronically implanted at the L6 and L7 DRG with penetrating multichannel microelectrode arrays. During each week of testing, we identified electrode channels that recruited primary afferent neurons with fast (80–120 m s−1) and medium (30–75 m s−1) conduction velocities, and selected one channel to deliver current-controlled biphasic stimulation trains during quiet standing. Main results. Postural responses were identified by changes in GRFs and were characterized based on their magnitude and latency. During DRG microstimulation, animals did not exhibit obvious signs of distress or discomfort, which could be indicative of pain or aversion to a noxious sensation. Across 56 total weeks, 13 electrode channels evoked behavioral responses, as detected by a significant change in GRF. Stimulation amplitude modulated the magnitude of the GRF responses for these 13 channels (p < 0.001). It was not possible to predict whether or not an electrode would drive a behavioral response based on information including conduction velocity, recruitment threshold, or the DRG in which it resided. Significance. The distinct and repeatable effects on the postural response to low amplitude (<40 µA) DRG microstimulation support that this technique may be an effective way to restore somatosensory feedback after neurological injuries such as amputation.