Period1 gene expression in the olfactory bulb and liver of freely moving streptozotocin-treated diabetic mouse
Period1 gene expression in the olfactory bulb and liver of freely moving streptozotocin-treated diabetic mouse
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DOI：
10.1016/j.bbrc.2021.04.049
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发表时间：
2021-05-06
影响因子：
3.1
通讯作者：
Hamada, Toshiyuki
中科院分区：
文献类型：
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作者：
Kanou, Harumi;Nagasawa, Kouki;Hamada, Toshiyuki
时钟基因在大多数器官中表达昼夜节律。这些节律在整个身体组织起来，由大脑中的视交叉上核(SCN)调节。这些时钟基因表达节律的紊乱是肥胖等疾病的风险因素。为了探讨时钟基因在糖尿病发生发展中的作用，我们研究了链脲佐菌素(STZ)诱导的高糖对肝脏Perio1(PER1)基因表达节律和大脑嗅球(OB)表达的影响。我们发现注射STZ后，在血糖含量较低的情况下，PER1在两个组织中的表达都急剧增加。在快速表达高峰后，PER1的表达没有节律。随着葡萄糖含量的增加，行为变得心律不齐。最后，我们成功地检测到在给药后第1天，即症状出现之前，PER1在小鼠毛囊中的表达增加。这些结果表明，STZ上调PER1的表达在糖尿病的加重中起重要作用。(C)2021 Elsevier Inc.保留所有权利。
Clock genes express circadian rhythms in most organs. These rhythms are organized throughout the whole body, regulated by the suprachiasmatic nucleus (SCN) in the brain. Disturbance of these clock gene expression rhythms is a risk factor for diseases such as obesity. In the present study, to explore the role of clock genes in developing diabetes, we examined the effect of streptozotocin (STZ)-induced high glucose on Period1 (Per1) gene expression rhythm in the liver and the olfactory bub (OB) in the brain. We found a drastic increase of Per1 expression in both tissues after STZ injection while blood glucose content was low. After a rapid expression peak, Per1 expression showed no rhythm. Associated with an increase of glucose content, behavior became arrhythmic. Finally, we succeeded in detecting an increase of Per1 expression in mice hair follicles on day 1 after STZ administration, before the onset of symptoms. These results show that elevated Per1 expression by STZ plays an important role in the aggravation of diabetes. (C) 2021 Elsevier Inc. All rights reserved.