SPERMIDINE AND SPERMINE IN RAT TISSUES AT DIFFERENT AGES
SPERMIDINE AND SPERMINE IN RAT TISSUES AT DIFFERENT AGES
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DOI：
10.1111/j.1748-1716.1964.tb10433.x
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发表时间：
1964-01-01
期刊：
影响因子：
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通讯作者：
SIIMES, M
中科院分区：
文献类型：
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作者：
JANNE, J;RAINA, A;SIIMES, M
以氨基黑为定量试剂，纸电泳法分离精胺，测定了3月龄雄性和雌性大鼠15种不同组织中精胺和精胺的浓度。随后测定了新生大鼠和1月龄、3月龄和9月龄雄性大鼠的六种组织中这些多胺的浓度随年龄的变化。在3月龄时，肝脏和胸腺中的多胺含量最多，每克湿重的精胺含量为900~1600摩尔，精胺的摩尔含量为700~900摩尔。未观察到明显的性别差异。在大多数组织中，亚精胺的摩尔浓度高于精胺。所研究的每个组织中的亚精胺含量都随着年龄的增长而下降，在生命的第一个月下降最明显。精胺含量一般在第一个月有所增加，然后基本保持不变或略有下降。因此，精胺/精胺的摩尔比在出生后立即最高，在肝脏中的摩尔比在9个月内从4.52下降到0.84。在大脑中，与研究的其他组织相比，在前9个月中，它从1.31上升到2.08。
Concentrations of spermidine and spermine in fifteen different tissues of male and female rats aged three months were determined, using amido black as the quantitative reagent, after paper electrophoretic separation of the amines. The changes in the concentrations of these polyamines in relation to age were followed by determinations from six tissues of newborn rats and of male rats aged 1, 3 and 9 months. At the age of three months the largest amounts of the polyamines were found in the liver and the thymus, 900 to 1600 m[mu]moles of spermidine and 700 to 900 m[mu]moles of spermine per g wet weight. No great sex differences were observed. In most tissues the molar concentration of spermidine was higher than that of spermine. The spermidine content in every tissue studied decreased with increasing age, the fall being most marked during the first month of life. The spermine content as a rule increased somewhat during the first month and then remained nearly constant or decreased slightly. Consequently, the molar ratio spermidine/spermine was highest immediately after birth, decreasing in the liver, for instance, from 4.52 to 0.84 during 9 months. In the brain, in contrast to the other tissues investigated, it increased from 1.31 to 2.08 during the first 9 months.