Slow polymerization of Mycobacterium tuberculosis FtsZ
Slow polymerization of Mycobacterium tuberculosis FtsZ
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DOI：
10.1128/jb.182.14.4028-4034.2000
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发表时间：
2000-07-01
影响因子：
3.2
通讯作者：
Borhani, DW
中科院分区：
文献类型：
--
作者：
White, EL;Ross, LJ;Borhani, DW
细胞分裂所必需的蛋白质。来自结核分枝杆菌的FtsZ已在大肠杆菌中表达并纯化。重组蛋白具有微管蛋白和其它FtsZ的典型GTdR活性。使用90度光散射研究FtsZ聚合。分枝杆菌蛋白质达到最大聚合比大肠杆菌慢得多（类似于10分钟）。coli FtsZ.解聚也缓慢发生，在大多数条件下需要1小时或更长时间。聚合需要Mg ~（2+）和GTP，聚合所需的FtsZ的最小浓度为3 μ M。电子显微镜显示聚合的M.结核病FtsZ由链组成，所述链缔合以形成平行原丝的有序聚集体。6-氨基-2，3-二氢-4-苯基-1H-吡啶并[4，3-B] [1，4]二氮杂卓-8-基氨基甲酸乙酯（SRI 7614）是由Southern Research Institute合成的微管蛋白聚合抑制剂，结核FtsZ聚合，抑制GTP水解，并减少FtsZ聚合物的数量和大小。
The essential cell division protein. FtsZ, from Mycobacterium tuberculosis has been expressed in Escherichia coli and purified. The recombinant protein has GTPase activity typical of tubulin and other FtsZs. FtsZ polymerization was studied using 90 degrees light scattering. The mycobacterial protein reaches maximum polymerization much more slowly (similar to 10 min) than E. coli FtsZ. Depolymerization also occurs slowly, taking 1 h or longer under most conditions. Polymerization requires both Mg2+ and GTP, The minimum concentration of FtsZ needed for polymerization is 3 mu M. Electron microscopy shows that polymerized M. tuberculosis FtsZ consists of strands that associate to form ordered aggregates of parallel protofilaments. Ethyl 6-amino-2,3-dihydro-4-phenyl-1H-pyrido [4,3-b] [1,4] diazepin-8-ylcarbamate (SRI 7614), an inhibitor of tubulin polymerization synthesized at Southern Research Institute, inhibits M. tuberculosis FtsZ polymerization, inhibits GTP hydrolysis, and reduces the number and sizes of FtsZ polymers.