HUMORAL AND CELLULAR-RESPONSES OF MICE TO INFECTION WITH A COLD-ADAPTED INFLUENZA-A VIRUS VARIANT
HUMORAL AND CELLULAR-RESPONSES OF MICE TO INFECTION WITH A COLD-ADAPTED INFLUENZA-A VIRUS VARIANT
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DOI：
10.1128/iai.38.1.218-225.1982
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发表时间：
1982-01-01
影响因子：
3.1
通讯作者：
TANNOCK, GA
中科院分区：
文献类型：
--
作者：
MAK, NK;ZHANG, YH;TANNOCK, GA
在鼻内接种甲型流感病毒冷适应（ca）变体、流感病毒 A/Ann Arbor/6/60-ca 或亲代病毒、流感病毒 A/Ann Arbor/6/60 后，在不同时间测量 CBA/H 小鼠中诱导的血清抗体反应和四种不同的细胞免疫反应（细胞毒性 T 细胞、迟发型超敏反应 T 细胞、自然杀伤细胞和细胞毒性巨噬细胞水平）。在最高病毒接种剂量（5 log10 50%组织培养感染剂量）下，两组小鼠肺部的四种细胞反应均达到高水平，并且在接种任一病毒后第20天检测到血清抗体滴度。然而，虽然亲代病毒在小鼠肺部发生了广泛的复制，但观察到 ca 变体的复制非常有限。从宏观上看，亲本病毒的感染引起严重的肺损伤，而接种 ca 变体的小鼠几乎不存在这种损伤。接种 2 至 5 log10 50% 组织培养感染剂量的亲本病毒可诱导高细胞毒性 T 细胞反应，而只有最高剂量的 ca 变体才会引起明显显着的细胞毒性 T 细胞反应。由于接种 5 log10 50% 组织培养感染剂量的 ca 变体引起了实质性的初级免疫反应，而没有明显的肺损伤，因此 ca 变体的无毒力可能主要与其在小鼠肺部有效复制的能力有限有关。
The serum antibody response and four different cellular immune responses (cytotoxic T cells, delayed-type hypersensitivity T cells, natural killer cells, and cytotoxic macrophage levels) induced in CBA/H mice were measured at different times after intranasal inoculation of a cold-adapted (ca) variant of influenza A virus, influenza virus A/Ann Arbor/6/60-ca, or the parental virus, influenza virus A/Ann Arbor/6/60. At the highest dose of virus inoculated (5 log10 50% tissue culture infective doses), all four cellular responses reached high levels in the lungs of both groups of mice, and serum antibody titers were detected on day 20 after inoculation of either virus. However, whereas extensive replication of the parental virus occurred in the mouse lungs, very limited replication of the ca variant was observed. Macroscopically, infection with the parental virus caused gross lung damage, whereas such damage was almost absent in mice inoculated with the ca variant. Inoculation of 2 to 5 log10 50% tissue culture infective doses of the parental virus induced high cytotoxic T-cell responses, whereas only the highest dose of the ca variant caused a clearly significant cytotoxic T-cell response. As an inoculum of 5 log10 50% tissue culture infective doses of the ca variant caused a substantial primary immune response without appreciable lung damage, the avirulence of the ca variant may be primarily related to its limited ability to replicate productively in mouse lungs.