SV2, A BRAIN SYNAPTIC VESICLE PROTEIN HOMOLOGOUS TO BACTERIAL TRANSPORTERS
SV2, A BRAIN SYNAPTIC VESICLE PROTEIN HOMOLOGOUS TO BACTERIAL TRANSPORTERS
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DOI：
10.1126/science.1519064
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发表时间：
1992-08-28
期刊：
影响因子：
56.9
通讯作者：
SCHELLER, RH
中科院分区：
文献类型：
--
作者：
BAJJALIEH, SM;PETERSON, K;SCHELLER, RH
突触囊泡蛋白2（SV 2）是一种特异性定位于神经元和内分泌细胞分泌囊泡的膜糖蛋白。 作为理解SV 2在神经分泌中的功能的第一步，分离并表征了编码SV 2的大鼠脑互补DNA（cDNA）。该cDNA的分析预测，SV 2含有12个跨膜结构域。蛋白质的NH 2-末端的一半显示出与转运糖、柠檬酸盐和药物的细菌蛋白质家族的显著氨基酸序列同一性。在COS细胞中的SV 2 cDNA的表达产生了高水平的SV 2样免疫反应性分布在一个网状和点状图案，这表明本地化的细胞内膜。其定位于囊泡，预测的膜拓扑结构，和已知的转运蛋白的序列同一性表明，SV 2是一个突触囊泡特异性转运蛋白。
Synaptic vesicle protein 2 (SV2) is a membrane glycoprotein specifically localized to secretory vesicles in neurons and endocrine cells. As a first step toward understanding the function of SV2 in neural secretion, a rat brain complementary DNA (cDNA) that encodes SV2 was isolated and characterized. Analyses of this cDNA predict that SV2 contains 12 transmembrane domains. The NH2-terminal half of the protein shows significant amino acid sequence identity to a family of bacterial proteins that transport sugars, citrate, and drugs. Expression of the SV2 cDNA in COS cells yielded a high level of SV2-like immunoreactivity distributed in a reticular and punctate pattern, which suggests localization to intracellular membranes. Its localization to vesicles, predicted membrane topology, and sequence identity to known transporters suggest that SV2 is a synaptic vesicle-specific transporter.