Improving single molecule force spectroscopy through automated real-time data collection and quantification of experimental conditions.
Improving single molecule force spectroscopy through automated real-time data collection and quantification of experimental conditions.
复制标题
通过自动实时数据收集和实验条件量化改进单分子力谱。
DOI：
10.1016/j.ultramic.2013.07.020
复制
发表时间：
2014
期刊：
影响因子：
2.2
通讯作者：
Marszalek,PiotrE
中科院分区：
文献类型：
--
作者：
Scholl,ZackaryN;Marszalek,PiotrE
单分子力谱（SMFS）的好处显然超过了挑战，包括小样本量、繁琐的数据收集以及在主观数据选择过程中引入人为偏见。通过原子力显微镜 (AFM) 实验数据收集过程的自动化，可以部分消除这些困难。自动数据收集还可以提供有关成功力延伸记录频率的信息。在这里，我们报告了一种用于 SMFS 的 AFM 实时数据收集自动化的方法。该方法使用希尔伯特变换来计算力延伸包络，然后计算力破裂事件的锐度，以实时区分可用记录（图 1A）和噪声记录（图 1B）。
The benefits of single molecule force spectroscopy (SMFS) clearly outweigh the challenges which include small sample sizes, tedious data collection and introduction of human bias during the subjective data selection. These difficulties can be partially eliminated through automation of the experimental data collection process for atomic force microscopy (AFM). Automated data collection can also provide information about the frequency of successful force extension recordings. Here, we report a methodology for automation of the real-time data collection for AFM for SMFS. This method uses a Hilbert transform to calculate the force-extension envelope and then calculates the sharpness of the force rupture events to discern usable recordings (Figure 1A) from noisy recordings (Figure 1B) in real-time.