Effect of acute alcohol ingestion prior to burn injury on intestinal bacterial growth and barrier function
Effect of acute alcohol ingestion prior to burn injury on intestinal bacterial growth and barrier function
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DOI：
10.1016/j.burns.2004.09.021
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发表时间：
2005-05-01
期刊：
影响因子：
2.7
通讯作者：
Choudhry, MA
中科院分区：
文献类型：
--
作者：
Kavanaugh, MJ;Clark, C;Choudhry, MA
我们实验室之前的研究表明，烧伤前急性酒精（EtOH）摄入会促进肠道细菌易位。本研究测试了酒精和烧伤大鼠肠道细菌易位增加是否由于肠道细菌过度生长。我们确定易位是否伴随着肠通透性和免疫细胞群的改变。用酒精灌胃大鼠（225-250 g），使其在烧伤或假性损伤前（体表总面积的25%）血液中EtOH水平达到100 mg/dl。受伤后两天。我们发现，烧伤前急性酒精摄入导致小肠细菌计数显著增加。肠道细菌数量的增加伴随着肠道通透性的显著增加。最后，免疫组织化学分析显示，与烧伤或假损伤大鼠相比，酒精和烧伤大鼠肠道中T细胞和树突状细胞的大量损失（p < 0.05）。总之，本文的结果表明，肠道细菌生长的增加以及肠道通透性和免疫状态的改变有助于在酒精和烧伤大鼠中观察到的细菌易位的增加。(c) 2004年Elsevier Ltd和ISBI。版权所有。
Previous studies from our laboratory have shown that acute alcohol (EtOH) ingestion prior to burn injury enhances intestinal bacterial translocation. This study tested if increased intestinal bacterial translocation in alcohol and burn injured rats is due to an overgrowth in intestinal bacteria. We determined if the translocation was accompanied with alterations in intestinal permeability and immune cell population. Rats (225-250 g) were gavaged with alcohol to achieve a blood EtOH level in the range of 100 mg/dl prior to burn or sham injury (25% total body surface area). Two days after injury. we found that acute alcohol ingestions prior to burn injury results in a significant increase in bacterial Counts in small intestine. The increase in intestinal bacterial Counts accompanied a significant increase in intestinal permeability. Finally, immunohistochemical analysis revealed a substantial (p < 0.05) loss of both T cell and dendritic cells in intestine of alcohol and burn injured rats compared with intestine of rats receiving either burn or sham injury. Altogether, results presented in this manuscript suggest that increase in intestinal bacterial growth along with alterations in intestinal permeability and immune status contribute to the increase in bacterial translocation observed in alcohol and burn injured rats. (c) 2004 Elsevier Ltd and ISBI. All rights reserved.