Carbon black nanoparticles promote the maturation and function of mouse bone marrow-derived dendritic cells
Carbon black nanoparticles promote the maturation and function of mouse bone marrow-derived dendritic cells
复制标题
DOI：
10.1016/j.chemosphere.2008.05.054
复制
发表时间：
2008-09-01
期刊：
影响因子：
8.8
通讯作者：
Kobayashi, Takahiro
中科院分区：
文献类型：
--
作者：
Koike, Eiko;Takano, Hirohisa;Kobayashi, Takahiro
包括炭黑（CB）纳米颗粒的颗粒物质可增强体内抗原相关炎症和免疫球蛋白产生。树突状细胞（DC）作为抗原呈递细胞（APC）是最有能力诱导免疫应答的细胞。本研究旨在确定CB纳米颗粒是否影响体外DC的成熟/活化和功能。用粒细胞巨噬细胞集落刺激因子（GM-CSF）诱导BALB/c小鼠骨髓细胞分化为DC。在培养的第8天，将BM衍生的DC（BMDC）暴露于直径为14 nm或56 nm的CB纳米颗粒24小时。通过流式细胞术测量主要组织相容性复合物（MHC）II类、DEC 205、CD 80和CD 86（BMDC的成熟/活化标志物）的表达。通过同种异体混合淋巴细胞反应（MLR）试验评价BMDC功能。CB纳米颗粒显著增加BMDC中DEC 205和CD 86的表达，并倾向于增加MHC II类和CD 80的表达;然而，未观察到尺寸依赖性效应。另一方面，BMDC介导的MLR被CB纳米颗粒显著增强，并且14 nm CB纳米颗粒的增强大于56 nm CB纳米颗粒。总之，CB纳米颗粒可以促进BMDC的成熟/活化和功能，这可能与其对过敏性疾病和/或反应的影响有关。此外，BMDC介导MLR可能是用于环境毒物的佐剂活性的体外筛选的有用测定。(C)2008爱思唯尔有限公司保留所有权利。
Particulate matter including carbon black (CB) nanoparticles can enhance antigen-related inflammation and immunoglobulin production in vivo. Dendritic cells (DC) as antigen-presenting cells (APC) are the most capable inducers of immune responses. The present study was designed to determine whether CB nanoparticles affect the maturation/activation and function of DC in vitro. DC were differentiated from bone marrow (BM) cells of BALB/c mice by culture with granulocyte macrophage colony stimulating factor (GM-CSF). At day 8 of culture, BM-derived DC (BMDC)' were exposed to CB nanoparticles with a diameter of 14 nm or 56 nm for 24 h. The expression of major histocompatibility complex (MHC) class II, DEC205, CD80, and CD86 (maturation/activation markers of BMDC) was measured by flow cytometry. BMDC function was evaluated by an allogeneic mixed lymphocyte reaction (MLR) assay. CB nanoparticles significantly increased the expression of DEC205 and CD86 in BMDC and tended to increase MHC class II and CD80 expression; however, a size-dependent effect was not observed. On the other hand, BMDC-mediated MLR was significantly enhanced by the CB nanoparticles and the enhancement was greater by 14 nm CB nanoparticles than by 56 nm CB nanoparticles. Taken together, CB nanoparticles can promote the maturation/activation and function of BMDC, which could be related to their effects on allergic diseases and/or responses. in addition, BMDC-mediated MLR might be useful assay for in vitro screening for adjuvant activity of environmental toxicants. (C) 2008 Elsevier Ltd. All rights reserved.