Design of a multiphase osteochondral scaffold. II. Fabrication of a mineralized collagen-glycosaminoglycan scaffold
Design of a multiphase osteochondral scaffold. II. Fabrication of a mineralized collagen-glycosaminoglycan scaffold
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DOI：
10.1002/jbm.a.32361
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发表时间：
2010-03-01
影响因子：
4.9
通讯作者：
Gibson, Lorna J.
中科院分区：
文献类型：
--
作者：
Harley, Brendan A.;Lynn, Andrew K.;Gibson, Lorna J.
本文是描述使用胶原蛋白-糖胺聚糖和磷酸钙技术设计和开发用于关节软骨缺损再生修复的骨软骨支架的系列论文中的第二篇。之前的论文描述了一种在不使用滴定剂的情况下系统改变和控制三重共沉淀胶原蛋白、糖胺聚糖和磷酸钙（CGCaP）纳米复合材料的化学成分的技术（并发作图）。本文描述了 (1) 从 CGCaP 悬浮液制造多孔三维支架，(2) 表征此类支架的微观结构和机械性能，以及 (3) 改性磷酸钙矿物相。该方法建立在先前的基础上，已证明能够改变 CGCaP 悬浮液的组成（磷酸钙质量分数在 0 至 80 wt% 之间），并能够生产其孔结构（平均孔径：50-1000 μm）、CaP 相化学（透钙磷石、磷酸八钙、磷灰石）和交联密度（因此机械性能和降解速率）可以独立变化的支架。 控制。本文描述的支架将多孔胶原蛋白-糖胺聚糖支架的理想生化特性和孔隙结构与磷酸钙生物材料的强度和直接骨粘合特性相结合，可以定制以满足骨科和再生医学的一系列应用的需求。 (C) 2009 Wiley periodicals, Inc. J Biomed Mater Res 92A: 1066-1077, 2010
This paper is the second in a series of papers describing the design and development of in osteochondral scaffold using collagen-glycosaminoglycan and calcium phosphate technologies engineered for the regenerative repair of articular cartilage defects. The previous paper described a technology (concurrent mapping) for systematic variation and control of the chemical composition of triple coprecipitated collagen, glycosaminoglycan and calcium phosphate (CGCaP) nanocomposites without Using titrants. This paper describes (1) fabricating porous, three-dimensional scaffolds from the CGCaP suspensions, (2) characterizing the microstructure and mechanical properties of such scaffolds, and (3) modifying the calcium phosphate mineral phase. The methods build on the previously, demonstrated ability to vary the composition of a CGCaP suspension (calcium phosphate mass fraction between 0 and 80 wt %) and enable the production of scaffolds whose pore architecture (mean pore size: 50-1000 mu m), CaP phase chemistry (brushite, octacalcium phosphate, apatite) and crosslinking density (therefore mechanical properties and degradation rate) can be independently controlled. The scaffolds described in this paper combine the desirable biochemical properties and pore architecture of porous collagen-glycosaminoglycan scaffolds with the strength and direct bone-bonding properties of calcium phosphate biomaterials in a manner that can be tailored to meet the demands of a range of applications in orthopedics and regenerative medicine. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res 92A: 1066-1077, 2010