Collagen Surfaces Modified with Photo-Cleavable Polyethylene Glycol-Lipid Support Versatile Single-Cell Arrays of Both Non-adherent and Adherent Cells
Collagen Surfaces Modified with Photo-Cleavable Polyethylene Glycol-Lipid Support Versatile Single-Cell Arrays of Both Non-adherent and Adherent Cells
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DOI：
10.1002/mabi.201400312
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发表时间：
2014-12-01
影响因子：
4.6
通讯作者：
Nagamune, Teruyuki
中科院分区：
文献类型：
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作者：
Yamahira, Shinya;Yamaguchi, Satoshi;Nagamune, Teruyuki
光响应材料上的细胞图案化是制备独特单细胞阵列的有前途的工具。然而，这种智能材料上的大多数传统单细胞阵列只能应用于贴壁细胞，并限制细胞功能，例如图案化粘合表面内的延伸和迁移。在这项研究中，使用可光裂解的聚乙二醇 (PEG)-脂质/胶原表面构建了一种适用于非贴壁细胞和贴壁细胞的多功能单细胞阵列。在这个单细胞阵列上，细胞的行为与其本机功能相似，不受图案化表面的限制。此外，以高通量方式进行细胞运动和形态变化的定量成像分析。
Cell patterning on photo-responsive materials are a promising tool for preparing unique single-cell arrays. However, most conventional single-cell arrays on such smart materials can be applied only to adherent cells and limit cellular functions such as extension and migration within the patterned adhesive surfaces. In this study, a versatile single cell array that works with both non-adherent and adherent cells was constructed using a photo-cleavable polyethylene glycol (PEG)-lipid/collagen surface. On this single-cell array, cells behaved similar to their native functions without limitation from the patterned surface. Furthermore, quantitative imaging analyses of cellular motility and morphological changes were performed in a high-throughput manner.