Innate immune system still works at diapause, a physiological state of dormancy in insects.
Innate immune system still works at diapause, a physiological state of dormancy in insects.
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DOI：
10.1016/j.bbrc.2011.06.015
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发表时间：
2011-07
影响因子：
3.1
通讯作者：
Akihiro Nakamura;K. Miyado;Youki Takezawa;N. Ohnami;Masahiro Sato;C. Ono;Yuichirou Harada;Keiichi Yoshida;N. Kawano;Seiya Kanai;M. Miyado;A. Umezawa
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文献类型：
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作者：
Akihiro Nakamura;K. Miyado;Youki Takezawa;N. Ohnami;Masahiro Sato;C. Ono;Yuichirou Harada;Keiichi Yoshida;N. Kawano;Seiya Kanai;M. Miyado;A. Umezawa
滞育在昆虫中最常见，是一种不同于其他类型的休眠（如冬眠）的生理休眠状态。它可以让昆虫在恶劣的环境中生存或延长寿命。一般来说，非滞育昆虫的幼虫、蛹或成虫具有先天免疫系统，可防止微生物侵入其体内；然而，尚不清楚该系统是否在滞育的休眠条件下起作用。本文报道了巨蚕蛾蛹滞育过程中先天细胞反应的发生。扫描电镜分析表明，从蛹的胸部区域分离的体液中存在两种主要类型的细胞。当将乳胶珠作为外源靶标微注射到蛹的内部部分时，观察到这些细胞的吞噬和包封，这是先天细胞反应的特征。即使在4°C的低温下，这些细胞的这种行为仍然被观察到。我们的研究结果表明，先天细胞反应可以在休眠状态下的滞育昆虫中起作用。
Diapause is most often observed in insects and is a physiologically dormant state different from other types of dormancy, such as hibernation. It allows insects to survive in harsh environments or extend longevity. In general, larval, pupal, or adult non-diapausing insects possess an innate immune system preventing the invasion of microorganisms into their bodies; however, it is unclear whether this system works under the dormant condition of diapause. We here report the occurrence of innate cellular reactions during diapause using pupae of a giant silkmoth,Samia cynthia pryeri. Scanning electron microscopic analysis demonstrated the presence of two major types of cells in the body fluid isolated from the thoracic region of a pupa. Phagocytosis and encapsulation, characteristics of innate cellular reactions, by these cells were observed when latex beads as foreign targets were microinjected into the internal portion of a pupa. Such behavior by these cells was still observed even when pupae were continuously chilled at 4 °C. Our results indicate that innate cellular reactions can work in diapausing insects in a dormant state.