RNase One Gene Isolation, Expression, and Affinity Purification Models Research Experimental Progression and Culminates with Guided Inquiry-based Experiments
RNase One Gene Isolation, Expression, and Affinity Purification Models Research Experimental Progression and Culminates with Guided Inquiry-based Experiments
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DOI：
10.1002/bmb.20247
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发表时间：
2009-01-01
影响因子：
1.4
通讯作者：
Bailey, Cheryl P.
中科院分区：
文献类型：
--
作者：
Bailey, Cheryl P.
这个新的生物化学实验室课程通过实验的进展，产生一个平台，指导探究为基础的实验移动。从原核基因组DNA中分离RNase One基因，表达为标记蛋白，亲和纯化，并测试活性和底物特异性。学生对材料和方法的详细解释和学期的海报会议达到高潮。实验计划考虑到从基因分离到酶测定所需的费用和时间。这种由导师指导和学生设计的实验相结合，是在生物化学实验室课程中进行指导性探究式学习的一种可管理的尝试，同时为单个实验提供了一个有凝聚力的故事和背景。
This new biochemistry laboratory course moves through a progression of experiments that generates a platform for guided inquiry-based experiments. RNase One gene is isolated from prokaryotic genomic DNA, expressed as a tagged protein, affinity purified, and tested for activity and substrate specificity. Student pairs present detailed explanations of materials and methods and the semester culminates in a poster session. Experimental plans take into account the expense and time required to move from gene isolation to enzyme assays. This combination of instructor-guided and student-designed experiments is a manageable foray into guided inquiry-based learning in a biochemistry laboratory course, while providing a cohesive story and context for individual experiments.