Changes in Histopathology, Enzyme Activities, and the Expression of Relevant Genes in Zebrafish (Danio rerio) Following Long-Term Exposure to Environmental Levels of Thallium
Changes in Histopathology, Enzyme Activities, and the Expression of Relevant Genes in Zebrafish (Danio rerio) Following Long-Term Exposure to Environmental Levels of Thallium
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DOI：
10.1007/s00128-017-2176-5
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发表时间：
2017-11-01
影响因子：
2.7
通讯作者：
Liu, Ji-Sheng
中科院分区：
文献类型：
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作者：
Hou, Li-Ping;Yang, Yang;Liu, Ji-Sheng
铊是一种稀土元素，但广泛分布于水环境中，对我们的健康构成潜在风险。本研究旨在探讨低剂量铊暴露对成年斑马鱼的生理反应、基因表达和相关酶活性的影响。评估的终点包括金属硫蛋白（MT）2和热休克蛋白HSP 70的mRNA表达;超氧化物歧化酶（SOD）和Na+/K+-ATP酶的酶活性;以及鳃、性腺和肝组织的组织病理学。结果表明，在100 ng/L的铊和MT 2的表达暴露于500 ng/L的铊后，HSP 70 mRNA的表达显着增加。与对照鱼相比，暴露于20和100 ng/L铊的斑马鱼肝脏SOD活性和鳃Na+/K+-ATP酶活性显著升高。鳃、肝脏和性腺组织表现出不同程度的损伤。总体结果表明，铊可能会导致毒性斑马鱼在环境相关的水溶液浓度。
Thallium is a rare-earth element, but widely distributed in water environments, posing a potential risk to our health. This study was designed to investigate the chronic effects of thallium based on physiological responses, gene expression, and changes in the activity of relevant enzymes in adult zebra fish exposed to thallium at low doses. The endpoints assessed include mRNA expression of metallothionein (MT)2 and heat shock protein HSP70; enzymatic activities of superoxide dismutase (SOD) and Na+/K+-ATPase; and the histopathology of gill, gonad, and liver tissues. The results showed significant increases in HSP70 mRNA expression following exposure to 100 ng/L thallium and in MT2 expression following exposure to 500 ng/L thallium. Significantly higher activities were observed for SOD in liver and Na+/K+-ATPase activity in gill in zebra fish exposed to thallium (20 and 100 ng/L, respectively) in comparison to control fish. Gill, liver, and gonad tissues displayed different degrees of damage. The overall results imply that thallium may cause toxicity to zebra fish at environmentally relevant aqueous concentrations.