SPECIFIC BINDING-SITES FOR ESTROGEN AT OUTER SURFACES OF ISOLATED ENDOMETRIAL CELLS
SPECIFIC BINDING-SITES FOR ESTROGEN AT OUTER SURFACES OF ISOLATED ENDOMETRIAL CELLS
复制标题
DOI：
10.1038/265069a0
复制
发表时间：
1977-01-01
期刊：
影响因子：
64.8
通讯作者：
SZEGO, CM
中科院分区：
文献类型：
--
作者：
PIETRAS, RJ;SZEGO, CM
雌激素在应答细胞中比在非靶细胞中更容易积累和保留1。与雌二醇和其他类固醇激素特异性相互作用的细胞质大分子2似乎介导激动剂转移到核染色质，在那里复合物被认为促进表型效应的表达3 -6。一般认为，激素被动扩散到决定细胞特异性反应的“细胞质”受体7。但一些实验表明，类固醇激素与生物膜的成分相互作用，并可能通过膜介导的过程进入其各自的靶细胞8 - 12，该过程是饱和的和温度依赖性的13 -17。我们研究了与子宫内膜、肝脏和肠粘膜分离的细胞质膜相关的类固醇结合成分。子宫内膜和肝细胞显示出与通过共价键固定在惰性支持物上的雌激素的大量结合，而肠细胞没有这样的结合位点。这些类固醇受体在不同组织细胞外表面的相对数量与给定细胞积累和保留雌激素的能力相对应。
OESTROGENS are more readily accumulated and retained in responsive cells than in cells that are not their targets1. Cytoplasmic macromolecules2which specifically interact with oestradiol and other steroid hormones seem to mediate transfer of the agonist to the nuclear chromatin, where the complex is believed to promote expression of the phenotypic effects3–6. It is generally assumed that the hormone diffuses passively to “cytoplasmic” receptors which determine the cellular specificity of response7. But some experiments indicate that steroid hormones interact with components of biological membranes and may enter their respective target cells by a membrane-mediated process8–12which is saturable and temperature-dependent13–17. We have investigated steroid-binding components associated with the plasma membranes of cells isolated from endometrium, liver and intestinal mucosa. Endometrial and liver cells show substantial binding to oestrogen immobilised by covalent linkage to an inert support, while intestinal cells have no such binding sites. The relative quantity of these steroid receptors at the outer surfaces of cells from diverse tissues corresponds well with the capacity of a given cell to accumulate and retain oestrogen.