C4 isoform of NADP-malate dehydrogenase. cDNA cloning and expression in leaves of C4, C3, and C3-C4 intermediate species of Flaveria.
C4 isoform of NADP-malate dehydrogenase. cDNA cloning and expression in leaves of C4, C3, and C3-C4 intermediate species of Flaveria.
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NADP-苹果酸脱氢酶的 C4 亚型。
DOI：
10.1104/pp.108.3.1119
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发表时间：
1995
期刊：
影响因子：
7.4
通讯作者：
Nelson,T
中科院分区：
文献类型：
--
作者：
McGonigle,B;Nelson,T
在NADP-苹果酸酶类型的C4植物中，一种丰富的、定位于叶肉细胞的NADP-苹果酸脱氢酶(MDH)作用于将固碳的初始产物草酰乙酸酯转化为苹果酸，然后将其穿梭到束鞘中。由于NADP-MDH在C3和C4植物的叶片中具有不同但重要的作用，我们克隆并鉴定了编码人NADP-MDH的全长cDNA，以便于在黄花属内进行结构/表达的比较研究。双子叶植物黄花属包括C3-C4中间种，以及C3和C4种。我们表明，先前提到的C3、C4和C3-C4黄萎菌种间NADP-MDH活性水平的差异部分是由于种间mRNA积累的差异。我们还发现NADP-MDH基因在不同的黄萎菌种之间似乎以单拷贝的形式存在，这表明在从C3植物到C4植物的进化过程中，一个先前存在的基因被重新调控，以适应C4循环的丰度和定位要求。
In C4 plants of the NADP-malic enzyme type, an abundant, mesophyll cell-localized NADP-malate dehydrogenase (MDH) acts to convert oxaloacetate, the initial product of carbon fixation, to malate before it is shuttled to the bundle sheath. Since NADP-MDH has different but important roles in leaves of C3 and C4 plants, we have cloned and characterized a nearly full-length cDNA encoding NADP-MDH from Flaveria trinervia (C4) to permit comparative structure/expression studies within the genus Flaveria. The dicot genus Flaveria includes C3-C4 intermediate species, as well as C3 and C4 species. We show that the previously noted differences in NADP-MDH activity levels among C3, C4, and C3-C4 Flaveria species are in part due to interspecific differences in mRNA accumulation. We also show that the NADP-MDH gene appears to be present as a single copy among different Flaveria species, suggesting that a pre-existing gene has been reregulated during the evolution from C3 to C4 plants to accommodate the abundance and localization requirements of the C4 cycle.