Mouse Hus1, a homolog of the Schizosaccharomyces pombe hus1+ cell cycle checkpoint gene.
Mouse Hus1, a homolog of the Schizosaccharomyces pombe hus1+ cell cycle checkpoint gene.
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DOI：
10.1006/geno.1999.5865
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发表时间：
1999-07
期刊：
影响因子：
4.4
通讯作者：
R. Weiss;C. F. Kostrub;T. Enoch;P. Leder
中科院分区：
文献类型：
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作者：
R. Weiss;C. F. Kostrub;T. Enoch;P. Leder
细胞周期检查点是一种调控机制，当DNA复制失败或DNA或纺锤体损伤等关键事件发生时，它可以阻止细胞周期或启动细胞程序性死亡。在裂变酵母中，细胞周期检查点对DNA复制阻滞和DNA损伤的反应需要hus1+基因。最近发现了哺乳动物中hus1+的同源物，在这里我们报道了对小鼠hus1的详细分析。分离出约4.2 kb的全长cDNA，编码32kda小鼠Hus1蛋白。测定了该基因的基因组结构和外显子-内含子边界序列，发现小鼠Hus1由9个外显子组成。小鼠Hus1被定位到11号染色体的近端，因此是小鼠突变生殖细胞缺陷的候选基因，它们被定位到相同的基因组区域。最后，发现小鼠Hus1在多种成年组织和胚胎发育的几个阶段均有表达。
Cell cycle checkpoints are regulatory mechanisms that arrest the cell cycle or initiate programmed cell death when critical events such as DNA replication fail to be completed or when DNA or spindle damage occurs. In fission yeast, cell cycle checkpoint responses to DNA replication blocks and DNA damage require the hus1+ gene. Mammalian homologs of hus1+ were recently identified, and here we report a detailed analysis of mouse Hus1. An approximately 4.2-kb full-length cDNA encoding the 32-kDa mouse Hus1 protein was isolated. The genomic structure and exon-intron boundary sequences of the gene were determined, and mouse Hus1 was found to consist of nine exons. Mouse Hus1 was mapped to the proximal end of chromosome 11 and is therefore a candidate gene for the mouse mutation germ cell deficient, which maps to the same genomic region. Finally, mouse Hus1 was found to be expressed in a variety of adult tissues and at several stages of embryonic development.