Symbiochlorum hainanensis gen. et sp. nov. (Ulvophyceae, Chlorophyta) isolated from bleached corals living in the South China Sea
Symbiochlorum hainanensis gen. et sp. nov. (Ulvophyceae, Chlorophyta) isolated from bleached corals living in the South China Sea
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DOI：
10.1111/jpy.12779
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发表时间：
2018-12-01
影响因子：
2.9
通讯作者：
Cheng, Hao
中科院分区：
文献类型：
--
作者：
Gong, Sanqiang;Li, Zhiyong;Cheng, Hao
光/扫描电子/透射显微镜为基础的形态学分析和多核苷酸序列为基础的分子系统发育分析被用来确定和评估的一个新的身份不明的绿色珊瑚分离漂白珊瑚生活在中国南海的系统发育位置。这种新的未鉴定的绿色藻是一种单细胞海洋藻，具有单核营养细胞和多个叶绿体，核状体。它能形成由细胞壁覆盖的不动孢子囊，并通过释放自孢子进行繁殖。这些特征与Ignatius和Pseudocaracium两个属的特征有很大不同。这两个属已被容纳在伊格内修斯分支。获得了核小亚基核糖体RNA基因（18 S rRNA）、核糖体RNA基因内转录间隔区2（ITS 2）和核酮糖-1，5-二磷酸羧化酶/加氧酶大亚基基因（rbcL，partial）的核苷酸序列，并与已发表的绿色藻序列进行了比较。形态学、超微结构和多核苷酸序列分析结果支持该新的未鉴定绿色藻在石莼纲中的定位。该新分离物被命名为海南共生绿菌（Symbiochlorum hainanensis gen.et sp. nov.）一个新的姐妹谱系伊格内修斯分支，石莼纲，绿藻门。
Light/scanning electron/transmission microscopy-based morphological analyses and multiple nucleotide sequences-based molecular phylogenetic analyses are used to identify and assess the phylogenetic position of a new unidentified green alga isolated from bleached corals living in the South China Sea. This new unidentified green alga is a unicellular marine alga and has uninucleate vegetative cells and multiple chloroplasts with a pyrenoid. It can form aplanosporangium covered by cell walls and reproduces by releasing autospore. These features differ substantially from those of the two genera Ignatius and Pseudocharacium. Those two genera have been accommodated in the Ignatius clade. Nucleotide sequences of the nuclear small subunit ribosomal RNA gene (18S rRNA), internal transcribed spacer 2 of ribosomal RNA gene (ITS2) and ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit gene (rbcL, partial) are obtained and compared with published green algal sequences. The results from the morphology, ultrastructure, and multiple nucleotide sequences data support the placement of the new unidentified green alga in Ulvophyceae. This new unidentified isolate is described as Symbiochlorum hainanensis gen. et sp. nov., a new sister lineage to the Ignatius clade, Ulvophyceae, Chlorophyta.