Structure of antibacterial peptide microcin J25: A 21-residue lariat protoknot
Structure of antibacterial peptide microcin J25: A 21-residue lariat protoknot
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DOI：
10.1021/ja036677e
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发表时间：
2003-10-15
影响因子：
15
通讯作者：
Ebright, RH
中科院分区：
文献类型：
--
作者：
Bayro, MJ;Mukhopadhyay, J;Ebright, RH
抗菌肽microcin J25（MccJ 25）通过结合并阻断细菌RNA聚合酶的核苷酸摄取通道来抑制细菌转录。已发表的共价和三维结构表明MccJ 25是一个21-残基环。在这里，我们表明，公布的共价和三维结构是不正确的，MccJ 25实际上是一个21个残基的“lactobacillus prototonde”，由一个8个残基的环状部分，然后由一个13个残基的线性部分，环回和线程通过环状部分组成。MccJ 25是第一个涉及主链-侧链酰胺键的lactobacillus原结的例子。
The antibacterial peptide microcin J25 (MccJ25) inhibits bacterial transcription by binding within, and obstructing, the nucleotide-uptake channel of bacterial RNA polymerase. Published covalent and three-dimensional structures indicate that MccJ25 is a 21-residue cycle. Here, we show that the published covalent and three-dimensional structures are incorrect, and that MccJ25 in fact is a 21-residue “lariat protoknot”, consisting of an 8-residue cyclic segment followed by a 13-residue linear segment that loops back and threads through the cyclic segment. MccJ25 is the first example of a lariat protoknot involving a backbone-side chain amide linkage.